Conditional activation of Pax6 in the developing cortex of transgenic mice causes progenitor apoptosis
Conditional activation of Pax6 in the developing cortex of transgenic mice causes progenitor apoptosis
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DOI：
10.1242/dev.02809
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发表时间：
2007-04-01
期刊：
影响因子：
4.6
通讯作者：
Stoykova, Anastassia
中科院分区：
文献类型：
--
作者：
Berger, Joachim;Berger, Silke;Stoykova, Anastassia
在发育过程中，Pax 6在大多数皮质放射状胶质祖细胞中以头外侧高到尾内侧低的梯度表达，并赋予它们神经原性。使用Cre/loxP为基础的方法，我们研究了条件激活的两个Pax 6亚型，Pax 6和Pax 6 -5a，对转基因小鼠的皮质生成的影响。我们发现Pax 6或Pax 6 -5a的激活抑制了发育中的皮质中的祖细胞增殖。在激活转基因Pax 6后，在不同发育阶段具有不同内源性Pax 6表达水平的特定祖细胞库在细胞周期进展和凋亡或神经元细胞命运的获得中显示出缺陷。这些结果为Pax 6在哺乳动物皮质生成中的复杂作用提供了新的证据。
During development, Pax6 is expressed in a rostrolateral-high to caudomedial-low gradient in the majority of the cortical radial glial progenitors and endows them with neurogenic properties. Using a Cre/loxP-based approach, we studied the effect of conditional activation of two Pax6 isoforms, Pax6 and Pax6-5a, on the corticogenesis of transgenic mice. We found that activation of either Pax6 or Pax6-5a inhibits progenitor proliferation in the developing cortex. Upon activation of transgenic Pax6, specific progenitor pools with distinct endogenous Pax6 expression levels at different developmental stages show defects in cell cycle progression and in the acquisition of apoptotic or neuronal cell fate. The results provide new evidence for the complex role of Pax6 in mammalian corticogenesis.