Assessing adhesion, biofilm formation and motility of Acidovorax citrulli using microfluidic flow chambers
Assessing adhesion, biofilm formation and motility of Acidovorax citrulli using microfluidic flow chambers
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DOI：
10.1111/j.1574-6968.2010.02094.x
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发表时间：
2010-11-01
影响因子：
2.1
通讯作者：
Burdman, Saul
中科院分区：
文献类型：
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作者：
Bahar, Ofir;De la Fuente, Leonardo;Burdman, Saul
瓜类细菌性果斑病是瓜类细菌性果斑病的病原菌。我们以前已经表明，IV型皮利（TFP）是野生型A.该病原菌能在甜瓜幼苗的木质部导管中定殖和移动。在这里，野生型和TFP突变株之间的比较研究，使用微流体流动室证明，TFP在表面附着和生物膜形成的A。西瓜在中等流量下。此外，TFP无效突变体不能进行逆着培养基流动方向的抽搐运动。使用鞭毛蛋白突变体的测定表明，与TFP相反，极性鞭毛不有助于A.在测试条件下的西瓜。此外，鞭毛介导的游泳运动的野生型菌株没有观察到介质流。这些结果意味着，TFP可能发挥重要的作用，在定殖和蔓延的木质部导管的液流条件下，而极性鞭毛可能是更重要的传播期间，当木质部流量最小的时间。
Acidovorax citrulli is the causal agent of bacterial fruit blotch of cucurbits. We have shown previously that type IV pili (TFP) are required for wild-type levels of virulence of A. citrulli on melon and that this pathogen can colonize and move thorough the xylem vessels of host seedlings. Here, comparative studies between wild-type and TFP mutant strains using microfluidic flow chambers demonstrated that TFP play a critical role in both the surface attachment and the biofilm formation of A. citrulli under a medium flow. Additionally, TFP null mutants were unable to perform twitching movement against the direction of medium flow. Assays using a flagellin mutant showed that, in contrast to TFP, polar flagella do not contribute to the adhesion and biofilm formation of A. citrulli under tested conditions. Also, flagellum-mediated swimming motility of wild-type strains was not observed under medium flow. These results imply that TFP may play an important role in colonization and spread in the xylem vessels under sap flow conditions, while polar flagella could be more important for spread during periods of time when xylem flow is minimal.