Bergamot (Citrus bergamia Risso) fruit extracts and identified components alter expression of interleukin 8 gene in cystic fibrosis bronchial epithelial cell lines.
Bergamot (Citrus bergamia Risso) fruit extracts and identified components alter expression of interleukin 8 gene in cystic fibrosis bronchial epithelial cell lines.
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DOI：
10.1186/1471-2091-12-15
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发表时间：
2011-04-15
期刊：
影响因子：
--
通讯作者：
Gambari R
中科院分区：
文献类型：
--
作者：
Borgatti M;Mancini I;Bianchi N;Guerrini A;Lampronti I;Rossi D;Sacchetti G;Gambari R
囊性纤维化（CF）气道病理学是一种致命的常染色体隐性遗传疾病，其特征是广泛的肺部炎症。在TNF-α诱导后，气道上皮细胞释放浓度升高的几种促炎细胞因子（即IL-6、IL-1β）和趋化因子（即IL-8）。为了减少CF患者气道中过度的炎症反应，人们开发了新的疗法，在这方面，人们研究了药用植物提取物。在这篇文章中，我们已经调查了佛手柑提取物（柑橘佛手柑Risso）和它们的鉴定成分的可能用途，以改变与囊性纤维化气道病理学相关的IL-8的表达。采用核磁共振氢谱（1H-NMR）、气相色谱-火焰离子化检测器（GC-FID）、气相色谱-质谱（GC-MS）和高压液相色谱（HPLC）对提取物进行了化学表征。对香柠檬提取物和主要检测化学成分进行了生物活性测定，测量（a）通过Bio-Plex细胞因子测定法测量用TNF-α处理的囊性纤维化IB 3 -1细胞培养上清中释放的细胞因子和趋化因子;（b）通过实时PCR测量IL-8 mRNA的积累。B。从佛手柑（Citrus bergamia Risso）外果皮获得的提取物含有对IL-8显示抑制活性的组分。特别是，最活跃的分子是佛手柑内酯和柠檬酸内酯。通过分析TNF-α诱导或暴露于热灭活铜绿假单胞菌的CF细胞模型IB 3 -1和CuFi-1中的mRNA水平和蛋白质释放，证实了这些作用。这些获得的结果清楚地表明，bergapten和citropten是IL-8表达的强抑制剂，并且可以被提议用于进一步的研究以验证可能的抗炎性质以减少CF患者中的肺部炎症。
Cystic fibrosis (CF) airway pathology is a fatal, autosomal, recessive genetic disease characterized by extensive lung inflammation. After induction by TNF-α, elevated concentrations of several pro-inflammatory cytokines (i.e. IL-6, IL-1β) and chemokines (i.e. IL-8) are released from airway epithelial cells. In order to reduce the excessive inflammatory response in the airways of CF patients, new therapies have been developed and in this respect, medicinal plant extracts have been studied. In this article we have investigated the possible use of bergamot extracts (Citrus bergamia Risso) and their identified components to alter the expression of IL-8 associated with the cystic fibrosis airway pathology. The extracts were chemically characterized by 1H-NMR (nuclear magnetic resonance), GC-FID (gas chromatography-flame ionization detector), GC-MS (gas chromatography-mass spectrometry) and HPLC (high pressure liquid chromatography). Both bergamot extracts and main detected chemical constituents were assayed for their biological activity measuring (a) cytokines and chemokines in culture supernatants released from cystic fibrosis IB3-1 cells treated with TNF-α by Bio-Plex cytokine assay; (b) accumulation of IL-8 mRNA by real-time PCR. The extracts obtained from bergamot (Citrus bergamia Risso) epicarps contain components displaying an inhibitory activity on IL-8. Particularly, the most active molecules were bergapten and citropten. These effects have been confirmed by analyzing mRNA levels and protein release in the CF cellular models IB3-1 and CuFi-1 induced with TNF-α or exposed to heat-inactivated Pseudomonas aeruginosa. These obtained results clearly indicate that bergapten and citropten are strong inhibitors of IL-8 expression and could be proposed for further studies to verify possible anti-inflammatory properties to reduce lung inflammation in CF patients.
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DOI：
10.1164/rccm.200207-765oc
发表时间：
2004-03-01
影响因子：
24.7
作者：
Becker, MN;Sauer, MS;Randell, SH
通讯作者：
Randell, SH
DOI：
10.1165/rcmb.2006-0200oc
发表时间：
2007-05-01
影响因子：
6.4
作者：
Dechecchi, Maria Cristina;Nicolis, Elena;Cabrini, Giulio
通讯作者：
Cabrini, Giulio
DOI：
10.1016/0165-1218(95)90070-5
发表时间：
1995-11-01
期刊：
MUTATION RESEARCH-GENETIC TOXICOLOGY
影响因子：
--
作者：
EDENHARDER, R;SPETH, C;PLATT, KL
通讯作者：
PLATT, KL
影响因子：
9.3
作者：
De Stefano, Daniela;De Rosa, Giuseppe;Carnuccio, Rosa
通讯作者：
Carnuccio, Rosa
DOI：
10.1165/ajrcmb.19.2.3053
发表时间：
1998-08-01
影响因子：
6.4
作者：
Black, HR;Yankaskas, JR;Noah, TL
通讯作者：
Noah, TL