Sperm-FISH analysis and human monitoring: a study on workers occupationally exposed to styrene
Sperm-FISH analysis and human monitoring: a study on workers occupationally exposed to styrene
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DOI：
10.1016/s1383-5718(03)00081-0
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发表时间：
2003-06-06
影响因子：
1.9
通讯作者：
Migliore, L
中科院分区：
文献类型：
--
作者：
Naccarati, A;Zanello, A;Migliore, L
目前正在调查职业接触苯乙烯这一在全世界广泛使用的化学品对人类健康包括男性生殖能力可能产生的有害影响。生殖细胞的非整倍体是不孕、流产和先天性疾病的主要原因。荧光原位杂交（FISH）是迄今为止分析精子染色体数目变化最有效的细胞遗传学分子技术，我们调查了职业接触苯乙烯的个体和健康对照组的非整倍体和二倍体频率。我们进行了多色FISH，使用特异性的性染色体和2号染色体的着丝粒区域的DNA探针，在去致密的精子核的样本与正常的精液参数，共18个苯乙烯暴露的受试者和13个未暴露的对照相同的年龄范围。受照者在过去5年中至少在生产增强塑料的工厂工作2年，并连续工作6个月。受检染色体的非整倍体和二倍体发生率在工人和对照组之间没有显示出统计学上的显著差异。接触苯乙烯与非吸烟者组性染色体缺体症的频率增加有关，尽管只有有限数量的受试者属于这一亚组。考虑到整个研究人群，年龄与XX二体性频率增加相关，而吸烟与性染色体减数分裂II不分离相关。总的来说，混杂因素似乎发挥更重要的影响比暴露于苯乙烯的精子核染色体数目的改变，选择正常的精液参数的主题。(C)2003 Elsevier Science B.V.保留所有权利。
Occupational exposure to styrene, a chemical extensively used worldwide, is under investigation for possible detrimental effects on human health, including male reproductive capacity. Aneuploidy in germ cells is the main cause of infertility, abortions and congenital diseases. Fluorescence in situ hybridisation (FISH), is the most efficient cytogenetic molecular technique to date to analyse numerical alterations of chromosomes in spermatozoa.We investigated the frequencies of aneuploidy and diploidy in individuals occupationally exposed to styrene and in healthy unexposed controls. We performed multicolour FISH, using DNA probes specific for the centromeric regions of sex chromosomes and chromosome 2, in decondensed sperm nuclei of samples with normal semen parameters for a total of 18 styrene-exposed subjects and 13 unexposed controls of the same age range. Exposed individuals had worked for at least 2 years during the last 5 years, and continuously for 6 months, in factories producing reinforced plastics.The incidence of aneuploidy and diploidy for the tested chromosomes did not show a statistically significant difference between workers and controls. The exposure to styrene was associated with increased frequencies of nullisomy for sex chromosomes in the group of non-smokers, although only a limited number of subjects belonged to this sub-group. Considering the whole study population, age was associated with an increased frequency of XX disomy, whereas smoking was associated with meiosis II non-disjunction of sex chromosomes. Overall, confounding factors appeared to exert a more important effect than exposure to styrene on numerical chromosome alterations in sperm nuclei of subjects selected for normal semen parameters. (C) 2003 Elsevier Science B.V. All rights reserved.