Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1
Isolation and characterization of the Chrysanthemum nitrate transporter CmNRT1
复制标题
菊花硝酸盐转运蛋白 CmNRT1 的分离和表征
DOI：
10.4238/gmr.15017148
复制
发表时间：
2016-01-01
影响因子：
0.4
通讯作者：
Chen, F. D.
中科院分区：
文献类型：
--
作者：
Gu, C. S.;Zhang, X. X.;Chen, F. D.
In this study, the nitrate transporter gene CmNRT1 was isolated from the chrysanthemum variety 'Nannongxuefeng'. The full-length cDNA contains an open reading frame of 1761 bp encoding 587 residues. Using qRT-PCR, we found that CmNRT1 was induced by 10 mM NO3- in roots and shoots. Two Arabidopsis thaliana transgenic plants expressing CmNRT1 were selected for functional analyses. Root N-15 influx in wild-type and transgenic A. thaliana lines under 10 or 0.2 mM (NO3)-N-15 was tested. Our results indicate that CmNRT1 encodes a constitutive component for a low-affinity transporter.
