Molecular analysis of the root canal microbiota associated with endodontic treatment failures
Molecular analysis of the root canal microbiota associated with endodontic treatment failures
复制标题
DOI：
10.1111/j.1399-302x.2007.00423.x
复制
发表时间：
2008-08-01
影响因子：
--
通讯作者：
Benno, Y.
中科院分区：
文献类型：
--
作者：
Sakamoto, M.;Siqueira, J. F., Jr.;Benno, Y.
根管治疗失败通常是由持续/继发性根内感染引起的，粪肠球菌被认为是主要的病原体。尽管如此，与牙髓治疗失败有关的细菌多样性的广度仍然需要通过与培养无关的方法不断探索。方法：采用16S核糖体RNA基因克隆文库分析根管治疗后根尖牙周炎牙根内菌群。结果：所有病例均检出细菌，证实了治疗后疾病的感染性病因。在9例病例中共发现6门74个细菌分类群。其中，55%被确定为尚未培养的种型，在许多情况下，这也构成了微生物群的很大比例。鉴定出25个新的种型。大多数牙齿有一个混合的联合体，平均每个病例有10个分类群。在不止一个案例中只发现了11个分类群，揭示了微生物群组成的高度个体间变异性。结论：目前的研究结果揭示了新的候选根管病原体，包括尚未培养的细菌和除粪肠杆菌外的分类群，它们可能参与治疗后根尖牙周炎相关的混合感染。
Introduction: The failure of endodontic treatment is usually caused by persistent/secondary intraradicular infections and Enterococcus faecalis has been considered to be the main pathogen involved. Nevertheless, the breadth of bacterial diversity involved with endodontic treatment failures remains to be consistently explored by culture-independent approaches.Methods: This study determined the intraradicular microbiota of root-canal-treated teeth with post-treatment apical periodontitis using 16S ribosomal RNA gene clone library analysis.Results: Bacteria were present in all cases, confirming the infectious etiology of post-treatment disease. Seventy-four bacterial taxa belonging to six phyla were found in the nine cases investigated. Of these, 55% were identified as as-yet-uncultivated phylotypes, which also made up a significant proportion of the microbiota in many cases. Twenty-five new phylotypes were identified. Most teeth harbored a mixed consortium, with a mean number of 10 taxa per case. Only 11 taxa were found in more than one case, revealing a high interindividual variability in the composition of the microbiota.Conclusion: The current findings revealed new candidate endodontic pathogens, including as-yet-uncultivated bacteria and taxa other than E. faecalis, which may participate in the mixed infections associated with post-treatment apical periodontitis.