The expression of several cell wall-related genes in Arabidopsis roots is down-regulated under boron deficiency
The expression of several cell wall-related genes in Arabidopsis roots is down-regulated under boron deficiency
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DOI：
10.1016/j.envexpbot.2007.12.004
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发表时间：
2008-05-01
影响因子：
5.7
通讯作者：
Gonzalez-Fontes, Agustin
中科院分区：
文献类型：
--
作者：
Camacho-Cristobal, Juan J.;Herrera-Rodriguez, Maria Begona;Gonzalez-Fontes, Agustin
植物细胞壁是一种动态结构，在植物的生长发育和对环境胁迫的反应中具有重要意义。有直接证据表明，硼(B)在鼠李糖醛酸Ⅱ(RG-II)与果胶组装的细胞壁交联中起作用，这表明B是细胞壁结构和功能所必需的。本工作的目的是研究缺硼对拟南芥根细胞壁相关基因表达的影响。为此，在添加2亩MB的营养液中水培生长植株，然后转移到无硼培养6和24 h，进行转录组分析，鉴定出几个与细胞壁相关的基因，这些基因的表达受到缺硼的下调。这些基因属于阿拉伯半乳糖蛋白、木葡聚糖内糖基转移酶/水解酶、多聚半乳糖醛酸酶、果胶裂解酶、果胶甲酯酶、膨胀素和纤维素合成酶等基因家族。这些结果表明，除了在细胞壁的结构组织中发挥重要作用外，B还可能直接或间接地影响与细胞壁相关的基因的表达水平。(C)2007 Elsevier B.V.保留所有权利。
Plant cell wall is a dynamic structure that is of fundamental importance in plant growth and development, as well as in the response to environmental stresses. There is direct evidence for a role of boron (B) in cross-linking of cell wall rhamnogalacturonan II (RG-II) and pectin assembly, which shows that B is essential for cell wall structure and function. The aim of the present work was to investigate the effects of B deficiency on the expression of cell wall-related genes in Arabidopsis roots. For this purpose, plants were grown hydroponically in a nutrient solution supplemented with 2 mu M B and then transferred to a boron-free medium for 6 and 24 h. A transcriptome analysis was carried out and several cell wall-related genes were identified, whose expressions were down-regulated by B deficiency. These genes belong to arabinogalactan protein, xyloglucan endotransglycosylase/hydrolase, polygalacturonase, pectate lyase, pectin methylesterase, expansin, and cellulose synthase-like gene families. These results suggest that in addition to the essential role that B plays in the structural organization of the cell wall, it could directly or indirectly have an impact on the expression levels of genes related to the cell wall. (C) 2007 Elsevier B.V. All rights reserved.