Asymmetric Organocatalysis and Analysis on a Single Microfluidic Nanospray Chip
Asymmetric Organocatalysis and Analysis on a Single Microfluidic Nanospray Chip
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DOI：
10.1002/anie.201102331
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发表时间：
2011-01-01
影响因子：
16.6
通讯作者：
Belder, Detlev
中科院分区：
文献类型：
--
作者：
Fritzsche, Stefanie;Ohla, Stefan;Belder, Detlev
将化学过程小型化到所谓的实验室芯片设备上在化学的不同领域中具有重要意义。除了增强的便携性、减少的试剂消耗和提高的安全性的优点之外，小型化平台的一个典型特征是实现更高的反应和分析速率的可能性。[1]微流体系统起源于分析科学，在过去十年中受到了广泛关注。目前，微流体在无机和有机化学中越来越受欢迎，因为合成是在基于芯片的微反应器[2]或基于毛细管的微流反应器上进行的。[3]虽然在微流控芯片装置中进行了各种反应并取得了令人印象深刻的结果，[4]但是，分析表征通常是通过常规的宏观仪器在芯片外进行的。然而，这种方法没有利用芯片技术的前景和全部潜力，即在一个单一设备上集成不同的功能，如化学合成和分析。因此，希望开发类似于微电子学的集成化学电路[5]作为新的化学工具，例如用于催化剂筛选[6]或用于生物过程的在线监测。[7]在以前的工作中，我们展示了第一种方法集成化学反应和分析在一个单一的微芯片上筛选对映选择性生物催化剂。[8]然而，该系统仅限于水性介质和天然荧光分子。[9]因此，我们打算开发一种先进的芯片系统，在合成化学中具有更广泛的适用性，包括利用非水反应介质和更通用的检测系统。在这种情况下，与质谱的耦合似乎非常有吸引力，[10]因为它为物质鉴定提供了额外的结构信息。
The miniaturization of chemical processes onto so-called labon-a-chip devices has gained significant importance in different fields of chemistry. Besides the advantages of enhanced portability, reduced reagent consumption, and improved safety, a characteristic feature of miniaturized platforms is the possibility to achieve higher reaction and analysis rates.[1] From their roots in analytical sciences, microfluidic systems have received much attention over the past decade. At present, microfluidics is becoming increasingly popular in inorganic and organic chemistry, as syntheses are performed on chip-based microreactors [2] or capillary-based microflow reactors.[3] While diverse reactions have been performed in microfluidic chip devices with impressive results,[4] the analytical characterization is, however, usually carried out off-chip by conventional macroscopic instruments. However, this approach does not exploit the promise and the full potential of chip technology, namely, the integration of different functionalities such as chemical synthesis and analysis on one single device. Thus, it is desirable to develop, in analogy to microelectronics, integrated chemical circuits [5] as new chemical tools, for example, for catalyst screening [6] or for online monitoring of biological processes.[7] In previous work we demonstrated a first approach for integrating chemical reactions and analysis on a single microchip for the screening of enantioselective biocatalysts.[8] Nevertheless, this system was limited to aqueous media and native fluorescent molecules.[9] Therefore we intended to develop an advanced chip system with a wider applicability in synthetic chemistry, including the utilization of non-aqueous reaction media and a more general detection system. In this context, the coupling to mass spectrometry appears to be very attractive,[10] as it provides additional structural information for substance identification.