STATISTICAL-METHODS FOR POLYPLOID RADIATION HYBRID MAPPING
STATISTICAL-METHODS FOR POLYPLOID RADIATION HYBRID MAPPING
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DOI：
10.1101/gr.5.2.136
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发表时间：
1995-09-01
期刊：
影响因子：
7
通讯作者：
LUNETTA, KL
中科院分区：
文献类型：
--
作者：
LANGE, K;BOEHNKE, M;LUNETTA, KL
辐射杂交定位是一种体细胞技术，用于排序沿染色体的遗传位点和估计相邻位点之间的物理距离。本文提出了一个模型的片段产生和保留的数据涉及两个或更多的拷贝感兴趣的染色体每克隆。这种多倍体数据可以通过初始照射正常的二倍体细胞或汇集单倍体或二倍体克隆来产生。目前的模型假设片段是在克隆的祖先细胞中根据沿每条染色体的独立泊松断裂过程产生的。一旦生成，片段将以相同的保留概率独立地保留在克隆中。在此基础上和较少限制的留存模型，统计标准，如最小义务中断，最大似然比和贝叶斯后验概率可以用来决定轨迹顺序。距离可以用最大似然法来估计。似然计算尤其具有挑战性，从隐马尔可夫链理论出发的计算技术证明是至关重要的。在这种情况下，可能包含输入错误。本文讨论的统计工具应用于人类4号染色体短臂上的14个位点。
Radiation hybrid mapping is a somatic cell technique for ordering genetic loci along a chromosome and estimating physical distances between adjacent loci. This paper presents a model of fragment generation and retention for data involving two or more copies of the chromosome of interest per clone. Such polyploid data call be generated by initially irradiating normal diploid cells or by pooling haploid or diploid clones. The current model assumes that fragments are generated in the ancestral cell of a clone according to an independent Poisson breakage process along each chromosome. Once generated, Fragments are independently retained in the clone with a common retention probability. On the basis of this and less restrictive retention models, statistical criteria such as minimum obligate breaks, maximum likelihood ratios, and Bayesian posterior probabilities can be used to decide locus order. Distances call be estimated by maximum likelihood. likelihood computation is particularly challenging, and computing techniques from the theory of hidden Markov chains prove crucial. Within this context it is possible to incorporate typing errors. The statistical tools discussed here are applied to 14 loci on the short arm of human chromosome 4.