MONOCLONAL-ANTIBODIES TO THE HUMAN INTERFERON-GAMMA RECEPTOR - BLOCKING OF THE BIOLOGICAL-ACTIVITIES OF INTERFERON-GAMMA AND PURIFICATION OF THE RECEPTOR
MONOCLONAL-ANTIBODIES TO THE HUMAN INTERFERON-GAMMA RECEPTOR - BLOCKING OF THE BIOLOGICAL-ACTIVITIES OF INTERFERON-GAMMA AND PURIFICATION OF THE RECEPTOR
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DOI：
10.1089/jir.1989.9.315
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发表时间：
1989-06-01
期刊：
影响因子：
--
通讯作者：
RUBINSTEIN, M
中科院分区：
文献类型：
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作者：
NOVICK, D;FISCHER, DG;RUBINSTEIN, M
抗人干扰素-γ的单克隆抗体（IFN-γ）通过给小鼠注射受体制剂来开发受体，所述受体制剂通过配体亲和色谱法从溶解的胎盘膜纯化。通过阻断125 I标记的IFN-γ结合的能力鉴定了三种抗体。与HeLa细胞上的受体在4 ℃下反应。C.这些抗体之一阻断IFN-γ的几种生物活性，包括其抗病毒活性、其诱导HLA-DR表面抗原的能力以及其保护细胞免受NK细胞介导的细胞毒性的能力。该抗体在37 ℃下显示出更高的细胞结合能力。C，并且被过量的IFN-γ显著较少地置换。与其他两种抗体相比。溶解的粗制胎盘膜制备物的免疫亲和层析产生纯化的受体，其表现出88，000的分子量。纯化的受体保留了其结合125 I标记的IFN-γ的能力。在溶液中。
Monoclonal antibodies against the human interferon-.gamma. (IFN-.gamma.) receptor wre developed by injecting mice with a preparation of receptor that was purified from solubilized placental membranes by ligand affinity chromatography. Three antibodies were identified by their ability to block the binding of 125I-labeled IFN-.gamma. to its receptor on HeLa cells at 4.degree. C. One of these antibodies blocked several biological activities of IFN-.gamma., including its antiviral activity, its ability to induce HLA-DR surface antigens, and its ability to protect cells from NK cell-mediated cytotoxicity. This antibody exhibited higher binding capacity to cells at 37.degree. C and was significantly less displaceable by an excess of IFN-.gamma. as compared with the other two antibodies. Immunoaffinity chromatography of solubilized crude placental membrane preparation yielded a purified receptor that exhibited a molecular weight of 88,000. The purified receptor retained its ability to bind 125I-labeled IFN-.gamma. in solution.