TRIPLET FORMATION AND TRIPLET DECAY IN REACTION CENTERS FROM THE PHOTOSYNTHETIC BACTERIUM Rhodopseudomonas sphaeroides
TRIPLET FORMATION AND TRIPLET DECAY IN REACTION CENTERS FROM THE PHOTOSYNTHETIC BACTERIUM Rhodopseudomonas sphaeroides
复制标题
光合细菌球形红假单胞菌反应中心的三联体形成和三联体衰变
DOI：
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发表时间：
1984
期刊：
影响因子：
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通讯作者：
M. Lutz
中科院分区：
文献类型：
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作者：
C. Schenck;P. Mathis;M. Lutz
摘要-在光合细菌的反应中心，随着初级泛醌的减少，初级电子供体(一对命名为P的细菌叶绿素)的三重态PR在几ns内被PO。用闪光吸收光谱法测定了几株球形葡萄球菌在反应中心温度对PR和3CAR形成和衰变动力学的影响。三重态能量转移的速率，用闪光后PR的衰变来测量，随着温度的降低而降低。在60~30K之间，能量转移的半衰期比3CAR的半衰期长(约6μS)，而在20K以下，能量转移慢于PR的内衰期(约100μS)。在某些情况下，很明显，PR和3Car独立衰变，不处于热平衡状态。类胡萝卜素向磷的单线态能量转移在所有温度下都是高效的。
Abstract— In the reaction center of photosynthetic bacteria, with the primary ubiquinone reduced, the triplet state PR of the primary electron donor (a pair of bacteriochlorophylls named P) is PO ulated with a takes place in a few ns. We measured by flash absorption spectroscopy the influence of temperature on formation and decay kinetics of PR and 3Car in the reaction center of several strains of R. sphaeroides. The rate of triplet energy transfer, measured as the decay of PR after a flash, decreases when the temperature is lowered. Between 60 and 30 K the half‐time of energy transfer becomes longer than the 3Car half‐time decay (about 6 μs) and below 20 K the transfer is slower than the internal decay of PR (about 100 μs). In several cases it is clear that PR and 3Car decay independently and are not in thermal equilibrium. The singlet energy transfer from carotenoid to P occurs with a high efficiency at all temperatures.