Artifacts in measuring absorption spectra of phytoplankton collected on a filter
Artifacts in measuring absorption spectra of phytoplankton collected on a filter
复制标题
测量过滤器上收集的浮游植物吸收光谱时的伪影
DOI：
10.4319/lo.1990.35.8.1804
复制
发表时间：
1990
影响因子：
4.5
通讯作者：
D. Stramski
中科院分区：
文献类型：
--
作者：
D. Stramski
I show a conventional filter technique for measuring absorption spectra of phytoplankton may be subject to errors arising from rapid decomposition of pigments collected on the filter. Incorrect values of spectral absorption indicating a higher proportion of degraded pigments relative to their actual cellular concentration can be measured by this method. The sensitivity of the method to these errors appears to be species specific. A simple refinement, incorporating chemical fixation of microorganisms before filtration, is proposed as a possible means to overcome these artifacts. Inadequacy of glass-fiber filters for retaining all absorbing particles is also suggested as a potential source of underestimation in particulate absorption measured traditionally on samples collected by filtration.
