An oral Brown Norway rat model for food allergy: comparison of age, sex, dosing volume, and allergen preparation
An oral Brown Norway rat model for food allergy: comparison of age, sex, dosing volume, and allergen preparation
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DOI：
10.1016/j.tox.2003.11.010
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发表时间：
2004-03-15
期刊：
影响因子：
4.5
通讯作者：
Madsen, C
中科院分区：
文献类型：
--
作者：
Pilegaard, K;Madsen, C
本实验的目的是研究在我们实验室中使用Brown Norway大鼠作为食物过敏模型的可能性。通过管饲法对雄性和雌性Brown Norway大鼠每天给药35天后，诱导抗卵清蛋白（OVA）的特异性血清IgE。研究了各种变应原制剂的影响：11级OVA、V级OVA和新鲜鸡蛋白色、年龄（4周龄与8周龄）、给药体积和动物供应商。一个普遍的发现是，女性的特异性IgE和IgG滴度和应答者数量在统计学上显著高于男性。鸡蛋白色制备、年龄、给药体积和动物供应商对中位IgE和IgG滴度以及应答者数量无统计学显著影响。雄性和雌性免疫反应之间的差异不能归因于每日摄入的OVA或通过肺部暴露的变化。在我们手中，口服棕色大鼠食物过敏模型产生了中等数量的IgE反应者，雄性和雌性分别为13-38和38-75%。在我们的实验室里，对这个模型进行进一步的实验，女性似乎是首选的性别。(C)2003爱思唯尔爱尔兰有限公司保留所有权利。
The purpose of the presented experiments was to study the possibility of using the Brown Norway rat as a model for food allergy in our laboratory. Specific serum IgE against ovalbumin (OVA) was induced after dosing male and female Brown Norway rats daily by gavage for 35 days. The influence of various preparations of allergen: OVA grade 11, OVA grade V, and fresh egg white, age (4 versus 8 weeks), dosing volumes, and animal suppliers was studied. A general finding was that females had statistically significantly higher specific IgE and IgG titres and number of responders than males. Egg white preparation, age, dosing volume, and animal supplier did not statistically significantly influence the median IgE and IgG titres and number of responders. The difference between immune responses in males and females could not be attributed to variations in daily intake of OVA or exposure via the lung. In our hands, the oral Brown rat food allergy model gives rise to a moderate number of IgE responders, 13-38 and 38-75% in males and females, respectively. For further experiments with this model in our laboratory, females seem the sex of choice. (C) 2003 Elsevier Ireland Ltd. All rights reserved.