Activated Müller Cells Involved in ATP-Induced Upregulation of P2X(7) Receptor Expression and Retinal Ganglion Cell Death.
Activated Müller Cells Involved in ATP-Induced Upregulation of P2X(7) Receptor Expression and Retinal Ganglion Cell Death.
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活化的 Muller 细胞参与 ATP 诱导的 P2X(7) 受体表达上调和视网膜神经节细胞死亡
DOI：
10.1155/2016/9020715
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发表时间：
2016
影响因子：
--
通讯作者：
Ji M
中科院分区：
文献类型：
--
作者：
Xue Y;Xie Y;Xue B;Hu N;Zhang G;Guan H;Ji M
P2X7 receptor (P2X7R), an ATP-gated ion channel, plays an important role in glaucomatous retinal ganglion cell (RGC) apoptotic death, in which activated retinal Müller glial cells may be involved by releasing ATP. In the present study, we investigated whether and how activated Müller cells may induce changes in P2X7R expression in RGCs by using immunohistochemistry and Western blot techniques. Intravitreal injection of DHPG, a group I metabotropic glutamate receptor (mGluR I) agonist, induced upregulation of GFAP expression, suggestive of Müller cell activation (gliosis), as we previously reported. Accompanying Müller cell activation, P2X7R protein expression was upregulated, especially in the cells of ganglion cell layer (GCL), which was reversed by coinjection of brilliant blue G (BBG), a P2X7R blocker. In addition, intravitreal injection of ATP also induced upregulation of P2X7R protein expression. Similar results were observed in cultured retinal neurons by ATP treatment. Moreover, both DHPG and ATP intravitreal injection induced a reduction in the number of fluorogold retrogradely labeled RGCs, and the DHPG effect was partially rescued by coinjection of BBG. All these results suggest that activated Müller cells may release ATP and, in turn, induce upregulation of P2X7R expression in the cells of GCL, thus contributing to RGC death.
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DOI：
10.1177/039463200702000210
发表时间：
2007-04-01
影响因子：
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