A Novel Nuclear Protein Phosphatase 2C Negatively Regulated by ABL1 is Involved in Abiotic Stress and Panicle Development in Rice
A Novel Nuclear Protein Phosphatase 2C Negatively Regulated by ABL1 is Involved in Abiotic Stress and Panicle Development in Rice
复制标题
ABL1 负调控的新型核蛋白磷酸酶 2C 参与水稻非生物胁迫和穗发育
DOI：
10.1007/s12033-012-9614-8
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发表时间：
2013-06-01
影响因子：
2.6
通讯作者：
Zhang, Hong-Sheng
中科院分区：
文献类型：
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作者：
Li, Yu-Sheng;Sun, Hui;Zhang, Hong-Sheng
2C型蛋白磷酸酶在植物逆境反应的信号转导中发挥着重要作用。在本文中，我们从水稻中鉴定出一个新的胁迫诱导的2C型蛋白磷酸酶基因OsSIPP2C1。OsSIPP2C1包含一个完整的1,074 bp的开放阅读框，编码357个氨基酸的蛋白质。OsSIPP2C1的表达在高盐、PEG6000和外源ABA的作用下上调，并且在正常、盐或干旱条件下在abl1突变体中增强。有趣的是，OsSIPP2C1 表达在穗发育早期增加。使用水稻原生质体细胞的亚细胞定位测定表明，OsSIPP2C1 主要位于细胞核中。总之，ABL1 负调控的核 PP2C 蛋白 OsSIPP2C1 参与了水稻的非生物胁迫和穗发育。
Type 2C protein phosphatase plays an important role in the signal transduction of stress response in plants. In this paper, we identified a novel stress-induced type 2C protein phosphatase geneOsSIPP2C1from rice.OsSIPP2C1contains a complete open reading frame of 1,074 bp, encoding a protein with 357 amino acids.OsSIPP2C1expression was up-regulated by high salt, PEG6000 and exogenous ABA, and enhanced in theabl1mutant under normal, salt, or drought condition. Interestingly,OsSIPP2C1expression was increased during the early panicle development. Subcellular localization assay using rice protoplast cells indicated that OsSIPP2C1 was predominantly located in the nucleus. Together, it is suggested that a nuclear PP2C protein OsSIPP2C1 negatively regulated by ABL1 is involved in abiotic stress and panicle development in rice.