Nonrandom loss of chromosome 15 in Syrian hamster tumours induced by v-Ha-ras plus v-myc oncogenes
Nonrandom loss of chromosome 15 in Syrian hamster tumours induced by v-Ha-ras plus v-myc oncogenes
复制标题
v-Ha-ras 加 v-myc 癌基因诱导的叙利亚仓鼠肿瘤中 15 号染色体非随机丢失
作者：
M. Oshimura;Gilmer Tm;Barrett Jc
在多种人类和动物肿瘤中观察到的非随机染色体重排1，2在某些情况下与细胞癌基因3，4的表达增强或放松调控有关。最近，研究表明，正常的二倍体啮齿动物细胞在两个协同的癌基因如myc+ras5-7转染后会发生肿瘤性转化。然而，将正常细胞转化为恶性细胞所需的步骤尚不清楚。如果两个癌基因的激活足以使肿瘤发生，则从由转基因癌基因转化的二倍体细胞产生的肿瘤可能保持二倍体或只有随机的染色体改变。我们对用v-Ha-ras加v-myc DNA或单独用多瘤DNA导入叙利亚仓鼠胚胎细胞后形成的肿瘤进行了细胞遗传学分析。多瘤诱导的肿瘤细胞是二倍体，而v-Ha-ras+v-myc癌基因诱导的肿瘤是单倍体，具有非随机的染色体改变，即15号染色体单体。因此，v-Ha-ras+v-myc癌基因诱导的肿瘤发生需要或有利于15号染色体的额外改变，即15号染色体的丢失。这些结果表明，在某些条件下，正常的二倍体细胞的肿瘤进展需要两个以上的步骤。
Nonrandom chromosome rearrangements, observed in a variety of human and animal tumours1,2, are associated in some cases with enhanced expression or deregulation of cellular oncogenes3,4. Recently, it was shown that normal, diploid rodent cells are neoplastically transformed following transfection with two cooperating oncogenes, for example myc plus ras5–7. However, the number of steps necessary to convert a normal cell into a malignant cell is unknown. If activation of two oncogenes is sufficient for tumorigenicity, tumours derived from diploid cells tranformed by the transfected oncogenes may remain diploid or have only random chromosome alterations. We have performed cytogenetic analyses of tumours formed after transfection of Syrian hamster embryo cells with either v-Ha-ras plus v-myc DNAs or polyoma DNA alone. Whereas polyoma-induced, tumour-derived cells were diploid, tumours induced by v-Ha-ras plus v-myc oncogenes were monoclonal and had a nonrandom chromosome change, monosomy of chromosome 15. Thus, an additional change, loss of chromosome 15, is required for or is advantageous for tumorigenicity induced by v-Ha-ras plus v-myc oncogenes. These results suggest that the neoplastic progression of normal, diploid cells requires more than two steps under certain conditions.
DOI：
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发表时间：
1982
期刊：
The Journal of biological chemistry
影响因子：
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作者：
White,BA;Bancroft,FC
通讯作者：
Bancroft,FC
肿瘤发生的染色体假说。
影响因子：
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作者：
Sandberg,AA
通讯作者：
Sandberg,AA