The complete amino acid sequences of both subunits of the sweet protein monellin.
The complete amino acid sequences of both subunits of the sweet protein monellin.
复制标题
甜蛋白莫内林两个亚基的完整氨基酸序列。
DOI：
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发表时间：
1976
期刊：
影响因子：
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通讯作者：
H. Zuber
中科院分区：
文献类型：
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作者：
G. Frank;H. Zuber
已经确定了甜蛋白Monellin两条链的氨基酸序列。由于链分离不容易完成，因此在唯一的蛋氨酸残基（b链）上进行了溴化氰裂解，并分离了溴化氰裂解后的三种产物。采用高效液相色谱法对氨基酸型苯硫代氢毒素进行鉴定。因此，在自动测序仪上对多肽进行Edman降解后，可以确定a链的44个残基中的37个和B链的大CNBr片段的42个残基中的40个。
The amino acid sequences of both chains of the sweet protein Monellin have been determined. Since chain separation could not be accomplished easily, cyanogen bromide cleavage at the only methionine residue (in the B-chain) was performed and the three products obtained after cyanogen bromide cleavage were separated. For the identification of amino acid phenylthiohydantoins, high performance liquid chromatography was employed. Thus 37 out of a total of 44 residues of the A chain and 40 out of a total of 42 residues of the large CNBr fragment of the B chain could be determined after Edman degradation of the polypeptides on an automated sequenator.