Effect of various proteinase inhibitors on ovulation of explanted hamster ovaries.
Effect of various proteinase inhibitors on ovulation of explanted hamster ovaries.
复制标题
各种蛋白酶抑制剂对外植仓鼠卵巢排卵的影响。
DOI：
10.1530/jrf.0.0680407
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
S. Murao
中科院分区：
文献类型：
--
作者：
S. Ichikawa;H. Morioka;M. Ohta;K. Oda;S. Murao
取发情前期21：00~24：00h摘除的金黄地鼠卵巢，用微生物蛋白水解酶抑制剂孵育至次日上午10：30h，检测其对排卵的阻断作用。在所研究的11种蛋白酶抑制剂中，金属蛋白酶的特异性抑制剂talopeptin和丝氨酸蛋白酶和硫醇酶的特异性抑制剂α-MAPI是最强的阻滞剂。这两种抑制物在阻止排卵方面表现出时间顺序上的差异。丝氨酸蛋白酶抑制剂S-SI、纤溶酶链球蛋白、弹性蛋白、止痛剂和糜蛋白酶抑制素部分但显著地抑制排卵。这些结果表明，除了先前报道的一种金属蛋白酶外，对α-MAPI敏感的一种蛋白水解酶在金黄地鼠的排卵过程中也是必不可少的，丝氨酸蛋白酶(S)也参与了金黄地鼠卵巢的排卵。
Hamster ovaries explanted at 21:00-24:00 h on the day of pro-oestrus were incubated with microbial proteinase inhibitors until 10:30 h on the next morning and the ovulatory blocking effect of these inhibitors was examined. Amongst 11 proteinase inhibitors examined, talopeptin, a specific inhibitor for metallo-proteinases, and alpha-MAPI, a specific inhibitor for serine and thiol proteinases, were the strongest blockers. These 2 inhibitors exhibited a chronological discrepancy in their blocking effect on ovulation. S-SI, plasminostreptin, elastatinal, antipain and chymostatin, which are inhibitors for serine proteinases, partly but significantly inhibited ovulation. The results suggest that, in addition to a metallo-proteinase reported previously, a proteinase which is sensitive to alpha-MAPI is essential for the ovulatory process, and that serine proteinase(s) also participate in ovulation of the hamster ovary.