A rapid fluorometric method for the estimation of DNA in cultured cells.
A rapid fluorometric method for the estimation of DNA in cultured cells.
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一种用于估计培养细胞中 DNA 的快速荧光方法。
DOI：
10.1016/0003-2697(81)90194-9
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发表时间：
1981
影响因子：
2.9
通讯作者：
Florini,JR
中科院分区：
文献类型：
--
作者：
Erwin,BG;Stoscheck,CM;Florini,JR
我们在这里提出了一个程序的DNA和蛋白质从同一等分试样的细胞裂解液的快速测量。将通过该方法获得的DNA估计值与使用Kissane和Robins方法的重复测定值进行比较（1）。DNA估计的最佳范围（1 - 20 μg）非常适合用于单个细胞培养物的部分提取物;剩余提取物仍可用于酶测定或其他平行测定。
We present here a procedure for the rapid measurement of both DNA and protein from the same aliquot of cell lysate. DNA estimates obtained by this method were compared to replicate determinations using the method of Kissane and Robins (1). The optimal range for the estimation of DNA (1 to 20 μg) is well suited for use with portions of extracts from individual cell cultures; the remainder of the extract remains available for enzyme assays or other parallel determinations.