Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm
Buckwheat (Fagopyrum esculentum Moench) low molecular weight seed proteins are restricted to the embryo and are not detectable in the endosperm
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DOI：
10.1016/j.plaphy.2005.08.002
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发表时间：
2005-09-01
影响因子：
6.5
通讯作者：
Kreft, I
中科院分区：
文献类型：
--
作者：
Licen, M;Kreft, I
荞麦（Fagopyrum esculentum Moench）蛋白质在营养方面具有重要意义，因为其必需氨基酸含量高且均衡，使其生物学价值远高于谷类蛋白质。我们通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）分析了荞麦胚乳和胚蛋白质的提取物。在胚乳蛋白质的电泳图谱上，检测到六条明显的条带。两条最明显的条带分子量（M.W.s）范围在50到60 kDa之间。已表明57 kDa的蛋白质不会与针对分子量在23到25 kDa之间的蛋白质所产生的抗体发生交叉反应。没有关于其他胚乳蛋白质致敏性的报道。在荞麦胚乳的电泳图谱上未检测到低分子量蛋白质。在这项研究中，我们证明了胚乳和胚组织中不同大小类别蛋白质的组织特异性存在。(c) 2005 Elsevier SAS。保留所有权利。
Buckwheat (Fagopyrum esculentum Moench) proteins are nutritionally important because of their high and balanced content of essential amino acids making their biological value much higher than that of cereal proteins. We analyzed extracts of buckwheat endosperm and embryo proteins by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SIDS-PAGE). On electropherograms of endosperm proteins, six intense bands were detected. Two most intense bands were in the range of molecular weights (M.W.s) from 50 to 60 kDa. Protein of 57 kDa has been shown not to cross-react against antibodies raised against proteins of M.W. ranging between 23 and 25 kDa. There are no reports about the allergenicity of other endosperm proteins. On the electropherogram of buckwheat endosperm no low M.W. proteins could be detected. In this study we have demonstrated the tissue specific presence of proteins of different size classes of the endosperm and embryo tissues. (c) 2005 Elsevier SAS. All rights reserved.