Isolation and analysis of candidate ascochyta blight defence genes in chickpea. Part I. Generation and analysis of an expressed sequence tag (EST) library
Isolation and analysis of candidate ascochyta blight defence genes in chickpea. Part I. Generation and analysis of an expressed sequence tag (EST) library
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DOI：
10.1016/j.pmpp.2005.08.003
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发表时间：
2005-05-01
影响因子：
2.7
通讯作者：
Pang, ECK
中科院分区：
文献类型：
--
作者：
Coram, TE;Pang, ECK
鹰嘴豆(Cier arietum L.)是世界上第三大豆类作物，但限制产量的一个主要因素是一种严重的破坏性真菌病害，名为ascochyta Rabiei(Pass.)Labrousse)。利用鹰嘴豆(ICC3996)抗赤霉病，但因农艺性状不佳而未被栽培的品系，构建了一个丰富的EST序列文库。该文库由1021个EST组成，在公共数据库中进行同源性搜索，其中571个(56%)与现有数据库条目有显著同源性。其中4%与防御相关，编码木质素和植物保卫素生物合成酶、病程相关蛋白、信号蛋白和可能的防御蛋白。这些Uniges可能参与鹰嘴豆对Ascochyta疫病的防御，并进行了详细的讨论。EST文库的建立代表了功能基因组学方法的第一步，该方法旨在阐明与子囊藻枯萎病相关的基因的功能及其作用途径。讨论了EST文库在基因芯片表达研究和分子定位中的应用，重点是培育具有持久和广谱抗性的鹰嘴豆品种。(C)2005爱思唯尔有限公司。保留所有权利。
Chickpea (Cicer arietinum L.) is the world's third-most important pulse crop, but a major limiting factor to production is a severe and destructive fungal disease known as ascochyta blight (Ascochyta rabiei (Pass.) Labrousse). A chickpea accession (ICC3996) resistant to ascochyta blight, but uncultivated due to poor agronomic characters, was used to generate an enriched library of EST sequences. The library, consisting of 1021 ESTs, was characterised by homology searches in public databases, where 571 (56%) showed significant homology to existing database entries. The ESTs were clustered and assembled into 516 unigenes, of which 4% were defence-related, encoding lignin and phytoalexin biosynthesis enzymes, pathogenesis-related proteins, signalling proteins, and putative defensive proteins. These unigenes may be involved in chickpea defence against ascochyta blight, and are discussed in detail. The generation of an EST library represents the first step in a functional genomics approach aimed at elucidating the function of genes involved in ascochyta blight resistance and the pathway of their action. Applications of the EST library to microarray expression studies and molecular mapping are discussed, with emphasis on the development of agronomically viable chickpea cultivars with durable and broad-spectrum resistance to ascochyta blight. (c) 2005 Elsevier Ltd. All rights reserved.