BIOSYNTHESIS OF SIDERAMINES IN FUNGI - FUSIGEN SYNTHETASE FROM EXTRACTS OF FUSARIUM-CUBENSE
BIOSYNTHESIS OF SIDERAMINES IN FUNGI - FUSIGEN SYNTHETASE FROM EXTRACTS OF FUSARIUM-CUBENSE
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DOI：
10.1016/0014-5793(73)80401-6
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发表时间：
1973-01-01
期刊：
影响因子：
3.5
通讯作者：
DIEKMANN, H
中科院分区：
文献类型：
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作者：
ANKE, H;ANKE, T;DIEKMANN, H
2. 材料和方法培养cubense Smith (Tii 133)，测定其菌丝重量和铁胺含量，并按前文所述进行放射性计数[2,3,6]。制备无细胞提取物时，用Biichner漏斗过滤菌丝体，用0.1 M T&buffer （PH 7.2）洗涤，用两倍量的缓冲液（含0.04 M巯基乙醇）重悬，用布兰森Sonifier B-12超声处理1.5分钟。在30 000 g离心后，沉积物被丢弃，上清作为粗提物。从细胞中分离核酸
2. Materials and methodsFusarium cubense Smith (Tii 133) was cultured, its mycelial weight and sideramine content measured, and the radioactivity counted as previously described [2, 3, 6]. For preparation of cell-free extracts, the mycelium was filtered through a Biichner funnel, washed with 0.1 M T&buffer (PH 7.2), resuspended in twice the amount of buffer (containing 0.04 M mercaptoethanol) and sonicated for 1.5 min (Branson Sonifier B-12). After centrifugation at 30 000 g, the sediment was discarded, the supernatant serving as a crude extract. Nucleic acids were separated from the