Dietary Tributyrin Supplementation Attenuates Insulin Resistance and Abnormal Lipid Metabolism in Suckling Piglets with Intrauterine Growth Retardation.
Dietary Tributyrin Supplementation Attenuates Insulin Resistance and Abnormal Lipid Metabolism in Suckling Piglets with Intrauterine Growth Retardation.
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DOI：
10.1371/journal.pone.0136848
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Wang T
中科院分区：
文献类型：
--
作者：
He J;Dong L;Xu W;Bai K;Lu C;Wu Y;Huang Q;Zhang L;Wang T
宫内生长迟缓（IUGR）与胰岛素抵抗和脂质紊乱有关。丁酸三丁酸酯（trityin， TB）是丁酸酯的前药，具有减轻机体代谢功能障碍的作用。在本研究中，我们研究了添加TB对IUGR新生儿仔猪胰岛素抵抗和脂质代谢的影响。选取8头正常出生体重新生儿仔猪（NBW）和16头IUGR新生儿仔猪，于第7天断奶，分别饲喂基础乳饲粮（NBW组和IUGR组）或基础乳饲粮中添加0.1%三丁酸甘油酯（IT组，IUGR仔猪）至第21天（n = 8）。测定脂质代谢和mRNA表达的相关参数。与NBW组相比，IUGR组仔猪血清胰岛素浓度升高（P < 0.05），肝脏HOMA-IR和总胆固醇、甘油三酯（TG）、非酯化脂肪酸（NEFA）升高（P < 0.05），肝脏酶活性（肝脂肪酶[HL]、脂蛋白脂肪酶[LPL]、总脂肪酶[TL]）和肝糖原浓度降低（P < 0.05）。添加TB降低了血清中胰岛素、HOMA-IR、低密度脂蛋白胆固醇和高密度脂蛋白胆固醇的浓度（P < 0.05），降低了肝脏中TG和NEFA的浓度（P < 0.05），提高了肝脏中HL、LPL和TL酶活性和糖原浓度（P < 0.05）。IUGR显著（P < 0.05）影响了肝脏胰岛素信号转导通路和肝脏脂肪生成通路（包括转录因子和核因子）mRNA的表达，而饲粮中添加TB可有效（P < 0.05）减弱IUGR对肝脏胰岛素信号转导通路和肝脏脂肪生成通路（包括转录因子和核因子）mRNA的表达。通过增加酶活性和上调mRNA表达，添加TB具有降低IUGR仔猪胰岛素抵抗和脂质代谢异常的治疗潜力，从而早期改善IUGR仔猪的代谢效率。
Intrauterine growth retardation (IUGR) is associated with insulin resistance and lipid disorder. Tributyrin (TB), a pro-drug of butyrate, can attenuate dysfunctions in body metabolism. In this study, we investigated the effects of TB supplementation on insulin resistance and lipid metabolism in neonatal piglets with IUGR. Eight neonatal piglets with normal birth weight (NBW) and 16 neonatal piglets with IUGR were selected, weaned on the 7th day, and fed basic milk diets (NBW and IUGR groups) or basic milk diets supplemented with 0.1% tributyrin (IT group, IUGR piglets) until day 21 (n = 8). Relative parameters for lipid metabolism and mRNA expression were measured. Piglets with IUGR showed higher (P < 0.05) concentrations of insulin in the serum, higher (P < 0.05) HOMA-IR and total cholesterol, triglycerides (TG), non-esterified fatty acid (NEFA) in the liver, and lower (P < 0.05) enzyme activities (hepatic lipase [HL], lipoprotein lipase [LPL], total lipase [TL]) and concentration of glycogen in the liver than the NBW group. TB supplementation decreased (P < 0.05) the concentrations of insulin, HOMA-IR, low-density lipoprotein cholesterol, and high-density lipoprotein cholesterol in the serum, and the concentrations of TG and NEFA in the liver, and increased (P < 0.05) enzyme activities (HL, LPL, and TL) and concentration of glycogen in the liver of the IT group. The mRNA expression for insulin signal transduction pathway and hepatic lipogenic pathway (including transcription factors and nuclear factors) was significantly (P < 0.05) affected in the liver by IUGR, which was efficiently (P < 0.05) attenuated by diets supplemented with TB. TB supplementation has therapeutic potential for attenuating insulin resistance and abnormal lipid metabolism in IUGR piglets by increasing enzyme activities and upregulating mRNA expression, leading to an early improvement in the metabolic efficiency of IUGR piglets.