MGAlignIt: a web service for the alignment of mRNA/EST and genomic sequences
MGAlignIt: a web service for the alignment of mRNA/EST and genomic sequences
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DOI：
10.1093/nar/gkg561
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发表时间：
2003-07-01
影响因子：
14.9
通讯作者：
Ranganathan, S
中科院分区：
文献类型：
--
作者：
Lee, BTK;Tan, TW;Ranganathan, S
剪接是一种生物学现象，其从转录物中去除非编码序列以产生适合翻译的成熟转录物。为了研究这种现象，基因的内含子-外显子排列的信息是必不可少的，通常通过将mRNA/EST序列与其同源基因组序列进行比对来获得。MGAlign是一种新的、快速的、记忆效率高的、实用的mRNA/EST和基因组序列比对方法。我们在这里介绍一个免费的Web服务，MGAlignIt（http：//origin.bic.nus.edu.sg/mgalign/mgalignit），基于MGAlign。除了比对本身之外，该Web服务还允许用户以图形方式有效地可视化比对，并对比对输出执行有限的分析。服务器还允许以多种形式保存对齐，包括图形和文本，适合于其他程序的进一步处理和分析。
Splicing is a biological phenomenon that removes the non-coding sequence from the transcripts to produce a mature transcript suitable for translation. To study this phenomenon, information on the intron-exon arrangement of a gene is essential, usually obtained by aligning mRNA/EST sequences to their cognate genomic sequences. MGAlign is a novel, rapid, memory efficient and practical method for aligning mRNA/EST and genome sequences. We present here a freely available web service, MGAlignIt (http://origin.bic.nus.edu.sg/mgalign/mgalignit), based on MGAlign. Besides the alignment itself, this web service allows users to effectively visualize the alignment in a graphical manner and to perform limited analysis on the alignment output. The server also permits the alignment to be saved in several forms, both graphical and text, suitable for further processing and analysis by other programs.