RATE OF INACTIVATION OF ADENYL-CYCLASE AND PHOSPHODIESTERASE - DETERMINANTS OF BRAIN CYCLIC-AMP
RATE OF INACTIVATION OF ADENYL-CYCLASE AND PHOSPHODIESTERASE - DETERMINANTS OF BRAIN CYCLIC-AMP
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DOI：
10.1016/0024-3205(74)90168-4
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发表时间：
1974-01-01
期刊：
影响因子：
6.1
通讯作者：
STAVINOHA, WB
中科院分区：
文献类型：
--
作者：
JONES, DJ;MEDINA, MA;STAVINOHA, WB
为了评估不同组织灭活方法的时间要求的影响，测定了大鼠脑中环磷酸腺苷的浓度。通过以下方法获得组织的固定：断头并去除脑并在液氮中冷冻;断头进入液氮;整个动物浸入液氮中; 1.5kW最大场强微波照射8秒;和5 kW最大场强微波照射2秒。这些研究的结果表明，随着脑腺苷酸环化酶和磷酸二酯酶失活时间的减少，环磷酸腺苷的水平逐渐降低。在1.5 kW和5 kW微波灭活后环磷酸腺苷的局部脑浓度的研究中，同样的相关性也很明显。它的结论是，5千瓦的最大场强微波照射是最迅速的手段，允许更准确地估计内源性环磷酸腺苷浓度的酶失活。它的使用提供了快速灭活，创伤最小化，并通过易于解剖方便了局部代谢物的研究。
In order to assess the effects of time requirements of different tissue inactivation methods, concentrations of cyclic adenosine monophosphate in rat brain were determined. Fixation of tissues was obtained by the following methods: decapitation with removal of brain and freezing in liquid nitrogen; decapitation into liquid nitrogen; whole animal immersion in liquid nitrogen; 1.5 kW maximal field strength microwave irradiation for 8 seconds; and, 5 kW maximal field strength microwave irradiation for 2 seconds. Results of these studies indicate that as the time is reduced for inactivation of brain adenyl cyclase and phosphodiesterase, levels of cyclic adenosine monophosphate become progressively lower. This same correlation is also evident in studies of regional brain concentrations of cyclic adenosine monophosphate after 1.5 kW and 5 kW microwave inactivation. It is concluded that 5 kW maximal field strength microwave exposure is the most rapid means of enzyme inactivation permitting a more accurate estimation of endogenous cyclic adenosine monophosphate concentrations. Its use offers rapid inactivation with minimization of trauma and facilities the study of regional metabolites through ease of dissection.