ANAEROBIC-NITRATE, SYMBIOTIC AND AEROBIC GROWTH OF RHIZOBIUM-JAPONICUM - EFFECTS ON CYTOCHROME P450, OTHER HEMOPROTEINS, NITRATE AND NITRITE REDUCTASES
ANAEROBIC-NITRATE, SYMBIOTIC AND AEROBIC GROWTH OF RHIZOBIUM-JAPONICUM - EFFECTS ON CYTOCHROME P450, OTHER HEMOPROTEINS, NITRATE AND NITRITE REDUCTASES
复制标题
DOI：
10.1016/0005-2728(72)90215-0
复制
发表时间：
1972-01-01
期刊：
影响因子：
--
通讯作者：
DANIEL, RM
中科院分区：
文献类型：
--
作者：
DANIEL, RM
1.1.大豆根瘤菌菌株505（威斯康星州）在空气或2%O2下的液体培养物中共生生长，也在需氧和厌氧条件下与硝酸盐（6 mM）共生。厌氧硝酸盐生长诱导的血红素蛋白模式类似于但不完全相同的固氮共生细菌（拟杆菌）。特别是诱导的细胞色素P450具有不同的性质。完整的拟杆菌和厌氧硝酸盐细胞显示出高水平的硝酸还原酶活性，分别为1490和1240μ mol × h−1× g− 1蛋白质;空气生长或空气硝酸盐生长的细胞中的硝酸还原酶活性较低，分别为330和190μ mol × h−1× g− 1蛋白质。仅在厌氧硝酸盐生长的细胞中检测到亚硝酸盐还原酶活性。它的结论是，硝酸盐呼吸是显着的厌氧硝酸盐生长的细胞，但不是在空气中的硝酸盐细胞或拟杆菌。拟杆菌的特征性血红蛋白模式似乎依赖于近厌氧，而不是硝酸盐的存在。
1.1.Rhizobium japonicumstrain 505 (Wisconsin) was grown symbiotically in soybean root nodules, in liquid culture under air or 2% O2, also aerobically and anaerobically with nitrate (6 mM).2.2. Anaerobic-nitrate growth induces a haemoprotein pattern similar to but not identical with that of N2-fixing symbiotic bacteria (bacteroids). In particular, the induced cytochrome P450 has different properties.3.3. Whole bacteroids and anaerobic-nitrate cells showed high levels of nitrate reductase activity, respectively 1490 and 1240μmoles × h−1× g−1protein; nitrate reductase activity in air-grown or air-nitrate-grown cells was low, respectively 330 and 190μmoles × h−1× g−1protein. Nitrite reductase activity was detected only in anaerobic-nitrate-grown cells.4.4. It is concluded that nitrate respiration is significant in anaerobic-nitrate-grown cells, but not in air-nitrate cells or bacteroids. The characteristic haemoprotein pattern of bacteroids seems dependent on near-anaerobiosis rather than the presence of nitrate.