Antigen otoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cells
Antigen otoxicity of Agaricus blazei mushroom organic and aqueous extracts in chromosomal aberration and cytokinesis block micronucleus assays in CHO-k1 and HTC cells
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DOI：
10.1016/j.tiv.2005.08.009
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发表时间：
2006-04-01
影响因子：
3.2
通讯作者：
Mantovani, MS
中科院分区：
文献类型：
--
作者：
Bellini, MF;Angeli, JPF;Mantovani, MS
姬松茸(Agaricus blazei，Ab.)因其药用价值而广为人知。从科学上讲，它已经就其保护遗传物质免受损害的能力进行了测试。我们检测了不同的有机提取物(甲醇提取物-ME、己烷提取物-HE和正丁醇提取物-BE)和一种抗体的水提取物(AE)，以观察它们诱导DNA损伤的能力及其保护作用。用CHO-K1细胞染色体畸变率(CA)、非药物代谢细胞(CHO-K1)和药物代谢细胞(HTC)细胞的胞质分裂阻断微核试验(CBMN)检测其遗传损伤。提取物不具有裂解活性，但具有抗裂解活性。在CA中，BE(105%)和AE(126%)的降幅最大。Be对CHO-K1细胞无遗传毒性，但对HTC细胞有遗传毒性。然而，BE具有抗遗传毒性，可降低HTC和CHO-K1细胞的微核率。这些结果表明，所有提取物都含有保护性物质，但在某些情况下，它们可能在代谢方面表现出遗传毒性效应。因此，这些发现值得人们在使用这种蘑菇时谨慎使用。(C)2005爱思唯尔有限公司。保留所有权利。
Agaricus blazei (Ab) has become popularly known for its medicinal properties. Scientifically, it has been tested with regard to its capacity to protect genetic material against damage. We examined different organic extracts (methanolic extract-ME, hexanic extract-HE and n-butanolic extract-BE) and an aqueous extract (AE) of Ab, for their capacity to induce DNA damage as well as for their protective effect. Genetic damage was determined by the chromosomal aberration assay (CA) in CHO-k1 cells for all extracts and the cytokinesis block micronucleus assay (CBMN) in non drug-metabolizing (CHO-k1) and drug-metabolizing (HTC) cell lines for extract BE only. The extracts did not show clastogenicity but showed anticlastogenicity. The greatest percent reduction obtained were with BE (105%) and AE (126%) treatments in CA. BE treatment did not display genotoxicity in CHO-k1, but was genotoxic in HTC. However, BE was shown to be antigenotoxic causing decreased micronucleus frequency in HTC and CHO-k1 cells. These results suggest that all the extracts contained protective substances, but in some cases they could show a genotoxic effect with regard to metabolism. Therefore, these findings warrant caution in the use of this mushroom by the population. (c) 2005 Elsevier Ltd. All rights reserved.