ANALYSIS OF SCHIZOSACCHAROMYCES-POMBE MITOCHONDRIAL-DNA REPLICATION BY 2-DIMENSIONAL GEL-ELECTROPHORESIS
ANALYSIS OF SCHIZOSACCHAROMYCES-POMBE MITOCHONDRIAL-DNA REPLICATION BY 2-DIMENSIONAL GEL-ELECTROPHORESIS
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DOI：
10.1007/bf00368008
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发表时间：
1994-06-01
期刊：
影响因子：
1.6
通讯作者：
STACHOW, C
中科院分区：
文献类型：
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作者：
HAN, ZY;STACHOW, C
利用Brewer和Fangman发展的二维脉冲场凝胶电泳技术对裂殖酵母ura4-294h(-)的线粒体全基因组进行了分析。基因组由平均大小为100kb的多聚体组成，对完整线粒体DNA(MtDNA)基因组的重叠限制性片段进行分析后，得到了简单的Y-2D凝胶图谱。在金黄色葡萄球菌mtDNA制剂中发现含有大的或多个单链区域的大的单链DNA分子或双链DNA分子。发现线粒体DNA单体的复制发生在两个方向上。在这些结果的基础上，提出了一种最符合滚圆模型的复制机制。
The entire mitochondrial genome of Schizosaccharomyces pombe ura4-294h(-) was analyzed by the 2D pulsed field gel electrophoresis technique developed by Brewer and Fangman. The genome consists of multimers with an average size of 100 kb and analysis of the overlapping restriction fragments of the complete mitochondrial DNA (mtDNA) genome resulted in simple Y 2D gel patterns. Large single-stranded DNA molecules or double-stranded DNA molecules containing large or numerous single-stranded regions were found in the S. pombe mtDNA preparation. The replication of mtDNA monomers was found to occur in either direction. On the basis of these results, a replication mechanism for S. pombe mtDNA that is most consistent with a rolling circle model is suggested.