Cross-reactive and Vaccine-Induced Antibody to an Emerging Swine-Origin Variant of Influenza A Virus Subtype H3N2 (H3N2v)
Cross-reactive and Vaccine-Induced Antibody to an Emerging Swine-Origin Variant of Influenza A Virus Subtype H3N2 (H3N2v)
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DOI：
10.1093/infdis/jis500
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发表时间：
2012-12-15
影响因子：
6.4
通讯作者：
Li, Yan
中科院分区：
文献类型：
--
作者：
Skowronski, Danuta M.;Janjua, Naveed Z.;Li, Yan
背景最近在美国发现了由甲型流感病毒亚型H3 N2（H3 N2 v）的一种新型猪源变体引起的感染病例，主要发生在儿童中。我们通过年龄估计H3 N2 v交叉反应抗体的水平，并评估季节性三价灭活流感疫苗（TIV），有或没有佐剂，是否可以增加血清保护。通过血凝抑制（HI）试验评估H3 N2 v抗体，对于一个子集，还通过微量中和试验评估H3 N2 v抗体。血清阳性率和血清保护定义为HI滴度>= 40，并与祖先和当代人类菌株的水平进行比较。该分析包括2010年秋季收集的1116份血清，相当于每十年约100份血清。还评估了136名年龄≥ 65岁儿童在接受2011-2012年裂解TIV（31人）、MF 59佐剂TIV（72人）或无佐剂亚单位TIV（79人）之前和之后的血清中疫苗诱导的抗体水平。甲型H3 N2流感病毒HI滴度≥ 40的总体流行率为25%。没有年龄
Background. Cases of infection due to a novel swine-origin variant of influenza A virus subtype H3N2 (H3N2v) have recently been identified in the United States, primarily among children. We estimate levels of cross-reactive antibody to H3N2v by age and assess whether seasonal trivalent inactivated influenza vaccine (TIV), with or without adjuvant, may increase seroprotection.Methods. Antibody to H3N2v was assessed by hemagglutination inhibition (HI) assay and, for a subset, also by microneutralization assay. Seroprevalence and seroprotection were defined as an HI titer of >= 40, and levels were compared with those for ancestral and contemporary human strains. The analysis included 1116 sera collected during fall 2010, corresponding to approximately 100 sera per decade of life. Vaccine-induced antibody levels were also assessed in sera from 136 children aged = 65 years before and after receipt of 2011-2012 split TIV (for 31 individuals), MF59-adjuvanted TIV (for 72), or unadjuvanted subunit TIV (for 79).Results. The overall prevalence of HI titers of >= 40 against A(H3N2) v was 25%. No children aged