Expression of the adrenomedullin gene in adipose tissue
Expression of the adrenomedullin gene in adipose tissue
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DOI：
10.1016/j.regpep.2005.07.006
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发表时间：
2005-12-15
影响因子：
--
通讯作者：
Nakao, K
中科院分区：
文献类型：
--
作者：
Nambu, T;Arai, H;Nakao, K
肾上腺髓质素（AM）是一种有效的血管舒张肽，最初从人嗜铬细胞瘤细胞中分离出来。本报告关注AM在脂肪组织中的表达和分泌。Northern blot分析显示AM mRNA在小鼠脂肪组织中有明显表达。脂肪组织的表达水平是肾脏的2.5-3.2倍。成熟脂肪细胞中AM mRNA水平是脂肪组织间质-血管部分的7.3倍。在成熟脂肪细胞培养中，培养基中AM肽浓度随时间增加。在人皮下脂肪组织中也检测到AM的表达。肥胖小鼠模型、高脂饲料喂养小鼠和ob/ob小鼠中脂肪AM表达显著升高。这些结果表明，脂肪组织，特别是成熟脂肪细胞，是体内AM的主要来源，脂肪细胞衍生的AM在肥胖中起病理生理作用。(c) 2005 Elsevier B.V.版权所有
Adrenomedullin (AM) is a potent vasodilating peptide originally isolated from human pheochromocytoma cells. This report concerns the expression and secretion of AM from adipose tissue. Northern blot analysis demonstrated marked expression of AM mRNA in mouse adipose tissue. Expression levels in adipose tissues were 2.5-3.2 times higher than in the kidney. AM mRNA level in mature adipocytes was 7.3 times higher than in the stroma-vascular fraction of adipose tissue. In mature adipocyte culture, time-dependent increase of AM peptide concentration in the culture medium was detected. AM expression was also detected in human subcutaneous adipose tissue. Adipose AM expression significantly increased in obesity mouse model, high-fat diet fed mice and ob/ob mice. These results suggest that adipose tissue, especially mature adipocytes, is major source of AM in the body, and that adipocyte-derived AM plays a pathophysiological role in obesity. (c) 2005 Elsevier B.V. All rights reserved.