Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size.
Bone Regeneration Using Dentin Matrix Depends on the Degree of Demineralization and Particle Size.
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DOI：
10.1371/journal.pone.0147235
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Asahina I
中科院分区：
文献类型：
--
作者：
Koga T;Minamizato T;Kawai Y;Miura K;I T;Nakatani Y;Sumita Y;Asahina I
本研究旨在探讨牙本质基质的粒径和脱矿程度对骨再生的影响。将拔出的人牙粉碎，根据颗粒大小分为3组； 200、500 和 1000 μm。每组根据脱矿程度分为3组；未脱矿牙本质（UDD）、部分脱矿牙本质基质（PDDM）和完全脱矿牙本质基质（CDDM）。将牙本质样本植入大鼠颅骨骨缺损中。 4 周和 8 周后，通过显微 CT 图像、组织形态学和免疫组织化学分析评估骨再生情况。成骨细胞在 UDD 和 DDM 上培养，使用电子显微镜评估细胞附着情况。显微CT图像和组织学观察表明，CDDM已大量吸收，而UDD则没有，并且两者均诱导很少的骨形成，而所有粒径的PDDM均诱导更多的新骨形成，尤其是1000μm。电子显微镜观察显示成骨细胞附着在 DDM 上，但不附着在 UDD 上。粒径较大的 PDDM 诱导显着的骨再生，可能是因为 PDDM 具有适合细胞附着的表面。它的吸收和骨形成之间可能存在微妙的平衡。 PDDM可以被认为是一种潜在的骨替代品。
This study aimed to examine the influence of particle size and extent of demineralization of dentin matrix on bone regeneration. Extracted human teeth were pulverized and divided into 3 groups according to particle size; 200, 500, and 1000 μm. Each group was divided into 3 groups depending on the extent of demineralization; undemineralized dentin (UDD), partially demineralized dentin matrix (PDDM), and completely demineralized dentin matrix (CDDM). The dentin sample was implanted into rat calvarial bone defects. After 4 and 8 weeks, the bone regeneration was evaluated with micro-CT images, histomorphometric and immunohistochemical analyses. Osteoblasts were cultured on UDD and DDM to evaluate the cell attachment using electron microscope. Micro-CT images and histological observation revealed that CDDM had largely resorbed but UDD had not, and both of them induced little bone formation, whereas all particle sizes of PDDM induced more new bone, especially the 1000 μm. Electron microscopic observation showed osteoblasts attached to DDM but not to UDD. PDDM with larger particle size induced prominent bone regeneration, probably because PDDM possessed a suitable surface for cell attachment. There might be an exquisite balance between its resorption and bone formation on it. PDDM could be considered as a potential bone substitute.