Efficient plant regeneration from seedling explants of two commercially important temperate eucalypt species -: Eucalyptus nitens and E-globulus
Efficient plant regeneration from seedling explants of two commercially important temperate eucalypt species -: Eucalyptus nitens and E-globulus
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DOI：
10.1016/s0168-9452(98)00221-0
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发表时间：
1999-01-25
期刊：
影响因子：
5.2
通讯作者：
Hamill, JD
中科院分区：
文献类型：
--
作者：
Bandyopadhyay, S;Cane, K;Hamill, JD
建立了两种重要的温带桉树光叶桉（Eucalyptusnitens）和欧洲桉（Eucalyptusnitens）的离体植株再生方法。globulus，已经开发了利用幼苗外植体的方法。在Murashige和Skoog（MS）基本营养培养基上培养后，从两个物种的单个子叶和下胚轴外植体获得高度再生的愈伤组织，所述培养基补充有30 g l（-1）蔗糖、5-10%（v/v）椰子水、0.8%琼脂、1 mg l（-1）α-萘乙酸（NAA）和0.5 mg l（-1）N-6苄基氨基嘌呤（BAP）。将愈伤组织转移到含有0.5 mg l（-1）NAA和1 mg l（-1）BAP的再生培养基上7-8周后观察到芽分化。在少数情况下，直接芽再生发生在这两个物种没有一个干预的愈伤组织阶段。尽管每个子叶外植体的平均芽数通常高于下胚轴外植体，但来自下胚轴切段的愈伤组织（30-35%）的植株再生频率高于子叶外植体（20-25%）。在E. nitens，从器官发生的愈伤组织表面产生。在E.球状体。两个品种的再生芽（30-40%）在含有吲哚-3-丁酸（IBA）的改良MS培养基上均可生根，并成功地将植株移栽到土壤中。(C)1999爱思唯尔科学爱尔兰有限公司保留所有权利。
A reliable and reproducible method for plant regeneration in vitro of two important temperate eucalypts, Eucalyptus nitens and E. globulus, has been developed which utilises seedling explants. Highly regenerative callus was obtained from individual cotyledon and hypocotyledon explants of both species following cultivation on Murashige and Skoog's (MS) basal nutrient medium supplemented with 30 g l(-1) sucrose, 5-10% (v/v) coconut water, 0.8% agar, 1 mg l(-1) alpha-naphthalene-acetic acid (NAA) and 0.5 mg l(-1) N-6 benzylaminopurine (BAP). Shoot differentiation was observed 7-8 weeks after transfer of callus onto regeneration medium containing 0.5 mg l(-1) NAA and 1 mg l(-1) BAP. In a few instances, direct shoot regeneration occurred without an intervening callus phase in both species. The frequency of plans regeneration was higher for callus derived from hypocotyl segments (30-35%) compared to cotyledonary explants (20-25%) though the average number of shoots per cotyledonary explant was generally higher than for hypocotyl explants. Somatic embryos were observed occasionally in E. nitens, arising from the surface of organogenic callus. Organised structures closely resembling somatic embryos were also observed in E. globulus. Regenerated shoots (30-40%) of both species could be rooted in modified MS media containing indole-3-butyric acid (IBA) and plantlets were successfully transferred to soil. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.