Determination of the content of rosmarinic acid by HPLC and analytical comparison of volatile constituents by GC-MS in different parts of Perilla frutescens (L.) Britt.
Determination of the content of rosmarinic acid by HPLC and analytical comparison of volatile constituents by GC-MS in different parts of Perilla frutescens (L.) Britt.
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DOI：
10.1186/1752-153x-7-61
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发表时间：
2013-04-01
影响因子：
--
通讯作者：
Chen H
中科院分区：
文献类型：
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作者：
Liu J;Wan Y;Zhao Z;Chen H
紫苏Perilla frutescens（L.）布里特。既是食用植物，又是传统的治疗感冒的常用药用植物。在中国南方广泛种植。在《本草》中记载为药的紫苏的解剖部位为：紫苏、紫苏叶和紫苏果，分别为干燥的茎、干燥的叶和干燥的成熟果实。迷迭香酸是紫苏、紫苏和紫苏果中的主要多酚类成分之一。P. frutescens的特征挥发油被认为是该草药的另一种基本成分，使其用途广泛。建立了一种简便、快速、准确的HPLC-DAD法，适用于紫苏、紫苏叶、紫苏叶中迷迭香酸的含量测定。从中国大陆8个地区和香港药材市场采集12批紫苏、12批紫苏叶和13批紫苏果实，对紫苏的质量进行评价。结果表明，紫苏中迷迭香酸的含量最高。某些宏观特征与迷迭香酸的浓度有关。采用气相色谱-质谱联用（GC-MS）对紫苏、紫苏叶和紫苏叶的挥发性成分进行了鉴定和比较。通过蒸汽蒸馏制备提取物。从紫苏、紫苏子和紫苏子中分别鉴定出12、17和9种化合物，分别占总峰面积的69.71%、50.54%和81.73%。为了更有效地比较紫苏、紫苏叶和紫苏果的成分，对紫苏叶中的主要成分进行了分析。这项工作提供了一种方法，通过该方法可以评估P. frutescens的各个部分的样品中的活性成分。这对有效开发利用紫珠植物资源进行临床应用和药物开发具有一定的参考价值。
Perilla frutescens (L.) Britt. is not only an edible plant but also a traditional medicinal plant commonly used for treating common cold. It is widely cultivated in southern China. The anatomical parts of P. frutescens that are recorded as medicines in the Chinese material medica are: Perillae Caulis, Perillae Folium and Perillae Fructus, which are the dried stems, the dried leaves and the dried ripe fruits, respectively. Rosmarinic acid is one of major polyphenolic ingredients found in all three Perillae Caulis, Perillae Folium and Perillae Fructus. The characteristic volatile oil of P. frutescens is believed to be another essential composition of the herb, giving its wide range of use. A simple, rapid and accurate HPLC-DAD method was set up, suitable for the assay of rosmarinic acid in Perillae Fructus, Perillae Folium and Perillae Caulis. 12 batches of Perillae Caulis, 12 batches of Perillae Folium and 13 batches of Perillae Fructus from 8 different regions of mainland China and Hong Kong herbal markets were collected for evaluating the quality of P. frutescens. Results showed that Perillae Folium typically had the highest content of rosmarinic acid. Certain macroscopic characteristics were related to the concentration of rosmarinic acid. The volatile components were identified and compared in Perillae Fructus, Perillae Folium and Perillae Caulis by gas chromatography–mass spectrometry (GC-MS). Extracts were prepared by steam distillation. Twelve, seventeen and nine compounds were identified and accounted for 69.71%, 50.54% and 81.73% of all identified peak areas in Perillae Caulis, Perillae Folium and Perillae Fructus respectively. The identified components were analyzed for comparison of Perillae Caulis, Perillae Folium and Perillae Fructus more effectively. This work provides a means by which samples of various parts of P. frutescens can be evaluated in terms of their pharmacologically active components. It should be of value in the efficient exploitation of P. frutescens plant material in clinical applications and drug development.