Delay of testicular differentiation in the B6.YDOM ovotestis demonstrated by immunocytochemical staining for müllerian inhibiting substance.
Delay of testicular differentiation in the B6.YDOM ovotestis demonstrated by immunocytochemical staining for müllerian inhibiting substance.
复制标题
通过苗勒管抑制物质的免疫细胞化学染色证明 B6.YDOM 卵睾中睾丸分化的延迟。
DOI：
10.1016/0012-1606(91)90240-4
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发表时间：
1991
影响因子：
2.7
通讯作者：
Donahoe,PK
中科院分区：
文献类型：
--
作者：
Taketo,T;Saeed,J;Nishioka,Y;Donahoe,PK
将小家鼠Y染色体置于C57 BL/6 J（B6）小鼠背景上，其XY子代（B6.YDOM）在胎儿期发育有卵巢或卵睾丸，但不发育正常睾丸。我们用苗勒管抑制物质（MIS）免疫细胞化学染色方法研究了B6.YDOMovostestis睾丸异常分化的个体发生。我们发现，B6.YDOMovotestis启动睾丸分化晚于对照B6睾丸。当通过将B6.YDOM雄性与SJL/J雌性杂交将YDOM转移到SJL/J小鼠背景中时，所有XY后代均发育正常睾丸。睾丸分化的开始与B6雄性胎仔处于相同的发育阶段。这些结果表明，睾丸分化延迟不是由于YDOM染色体本身的作用，而是由于YDOM染色体上的睾丸决定基因与B6的常染色体基因的不适当的相互作用。此外，我们发现在B6.YDOMovostestis中，在精子原细胞前阶段的生殖细胞的停滞与邻近体细胞的MIS产生之间存在密切的相关性。这一结果可能支持MIS参与生殖细胞分化调控的假设。
It has been found that when the Y chromosome fromMus musculus domesticus(YDOM) is placed onto the C57BL/6J (B6) mouse background, the XY progeny (B6.YDOM) develop ovaries or ovotestes but not normal testes during fetal life. We examined the ontogeny of the abnormal testicular differentiation in the B6.YDOMovotestis by immunocytochemical staining for Müllerian inhibiting substance (MIS). We found that the B6.YDOMovotestis initiated testicular differentiation later in development than did the control B6 testis. When the YDOMwas transferred onto the SJL/J mouse background by crossing B6.YDOMmales with SJL/J females, all XY progeny developed normal testes. The onset of testicular differentiation was at the same developmental stage as in the B6 male fetus. These results suggest that the delay of testicular differentiation is not due to the effect of the YDOMchromosome itself, but due to improper interaction of the testis-determining gene on the YDOMchromosome with autosomal genes of B6. In addition, we found a close correlation between the arrest of germ cells at the prespermatogonia stage and MIS production of adjacent somatic cells in the B6.YDOMovotestis. This result may support the hypothesis that MIS is involved in the regulation of germ cell differentiation.