Replication potentials of vif variant viruses generated from monkey cell-tropic HIV-1 derivative clones NL-DT5/NL-DT5R
Replication potentials of vif variant viruses generated from monkey cell-tropic HIV-1 derivative clones NL-DT5/NL-DT5R
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DOI：
10.1016/j.micinf.2008.06.007
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发表时间：
2008-08-01
影响因子：
5.8
通讯作者：
Nomaguchi, Masako
中科院分区：
文献类型：
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作者：
Hatcho, Kazuki;Kamada, Kazuya;Nomaguchi, Masako
为了获得比原始NL-DT 5/NL-DT 5 R克隆更接近HIV-1的猴嗜性病毒，我们构建了六个vif嵌合病毒和两个位点特异性vif突变病毒，并检查它们的生长能力。与NL-DT 5/NL-DT 5 R不同，这些病毒在猴细胞中不生长。我们通过单循环感染性测定监测突变体拮抗猴APOBEC 3G/F的能力。它们对APOBEC 3G/F的作用抵消很差或根本没有抵消。我们的研究结果表明，天然SIVmac Vif是克服HIV-1的物种障碍所必需的。(c)2008年，Elsevier Masson SAS。All rights reserved.
To obtain monkey-tropic viruses that are more closely related to HIV-1 than the original NL-DT5/NL-DT5R clones, we constructed six vif-chimeric and two site-specific vif-mutant viruses, and examined their growth ability. Different from NL-DT5/NL-DT5R, these viruses did not grow in monkey cells. We monitored the capability of the mutants to antagonize monkey APOBEC3G/F by single-cycle infectivity assays. They counteracted poorly or not at all the action of the APOBEC3G/F. Our results have indicated that the native SIVmac Vif is required to overcome the species barrier against HIV-1. (c) 2008 Elsevier Masson SAS. All rights reserved.