Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater
Extended spectrum beta-lactamases in Escherichia coli from municipal wastewater
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DOI：
10.5604/12321966.1167710
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发表时间：
2015-01-01
影响因子：
1.7
通讯作者：
Kmet, Vladimir
中科院分区：
文献类型：
--
作者：
Cornejova, Tatiana;Venglovsky, Jan;Kmet, Vladimir
引言和目标。在过去的几十年里，人们对耐药生物的环境负荷的认识有所提高。本文对城市污水处理厂分离的产超广谱β-内酰胺酶的大肠埃希菌的耐药性、耐药基因的检测及产超广谱β-内酰胺酶的生物被膜情况进行了评价。以废水样品和人工添加的聚苯乙烯颗粒为来源进行大肠杆菌的分离。根据CLSI(2013)确定19种抗生素的最低抑菌浓度。用结晶紫法或重天青法研究生物膜的形成。用聚合酶链式反应检测CTX-M、碳青霉烯酶、qnrS、移动因子和毒力因子。用MALDI生物分型仪主成分分析检测菌株的克隆亲缘关系。在26%的环境菌株中检出ESBL表型。检测耐药基因CTX-M、CMY-2和qnrS。在一株耐厄他培南的大肠埃希菌中发现了imp基因和整合子1。细菌的耐药性、毒力与生物被膜的产生无明显相关性。结果表明，该废水是产超广谱β-内酰胺酶、碳青霉烯酶和对氟喹诺酮类药物耐药的主要来源。产生生物被膜的菌株、CTX-M组的耐药性、CMY-2、qnrS基因和毒力因子是潜在的环境健康风险。
Introduction and objective. Over the past decades, awareness of the environmental load of resistant organisms has increased. The presented paper focuses on antibiotic resistance and detection of resistance genes in environmental E. coli and on the evaluation of biofilm formation in ESBLs (extended spectrum beta-lactamase) producing E. coli isolated from an urban wastewater treatment plant.Materials and method. Wastewater samples and artificially added polystyrene pellets were used as the source for E. coli isolation. Minimal inhibitory concentrations of 19 antibiotics were determined according to CLSI (2013). Biofilm formation was investigated by crystal violet or resazurin methods. CTX-M, carbapenemases, qnrS, mobile elements and virulence factors were determined by PCR. Clonal relatedness of strains was detected by principal component analysis by a Maldi biotyper.Results. ESBL phenotype was detected in 26% of environmental strains. CTX-M, CMY-2 and qnrS genes of antibiotic resistance were detected. IMP gene together with integron 1 in one ertapenem resistant E. coli was also recorded. There was no evident correlation between antibiotic resistance, virulence and biofilm production.Conclusions. The results showed that the wastewater is a source of ESBLs, carbapenemases and plasmid fluoroquinolone resistance. Strains with biofilm production, antibiotic resistance of CTX-M group, CMY-2, qnrS genes and virulence factors present a potential environmental health risk.