Molecular hydrogen as an energy source for Helicobacter pylori
Molecular hydrogen as an energy source for Helicobacter pylori
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DOI：
10.1126/science.1077123
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发表时间：
2002-11-29
期刊：
影响因子：
56.9
通讯作者：
Maier, RJ
中科院分区：
文献类型：
--
作者：
Olson, JW;Maier, RJ
已知胃病原体幽门螺杆菌在实验室中生长时能够使用分子氢作为呼吸底物。我们发现氢在小鼠胃粘膜中是可利用的，并且它的使用大大增加了H.幽门。H.幽门螺杆菌是组成型的，但在与氢孵育后增加五倍。在活体小鼠胃中测得的氢浓度是H。幽门螺杆菌对氢的亲和力。氢化酶突变株在小鼠中的定殖效率要低得多。因此，氢存在于动物作为正常的结肠的结果。ORA是一种产生能量的底物，可以促进病原菌的维持。
The gastric pathogen Helicobacter pylori is known to be able to use molecular hydrogen as a respiratory substrate when grown in the laboratory. We found that hydrogen is available in the gastric mucosa of mice and that its use greatly increased the stomach colonization by H. pylori. Hydrogenase activity in H. pylori is constitutive but increased fivefold upon incubation with hydrogen. Hydrogen concentrations measured in the stomachs of live mice were found to be 10 to 50 times as high as the H. pylori affinity for hydrogen. A hydrogenase mutant strain is much less efficient in its colonization of mice. Therefore, hydrogen present in animals as a consequence of normal colonic. ora is an energy-yielding substrate that can facilitate the maintenance of a pathogenic bacterium.