Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick
Expression and function of microRNA-9 in the mid-hindbrain area of embryonic chick
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DOI：
10.1186/s12861-017-0159-8
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发表时间：
2018-02-22
影响因子：
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通讯作者：
Wizenmann, Andrea
中科院分区：
文献类型：
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作者：
Anand, A. Alwin Prem;Huber, Carola;Wizenmann, Andrea
背景：miR-9是一种小的非编码RNA，在物种之间高度保守，主要在中枢神经系统（CNS）中表达。已知其影响不同脊椎动物的脑和脊髓中的增殖和神经元分化。不同的研究表明，神经前体细胞对miR-9的反应存在区域性和种属特异性差异。方法：采用卵内和卵外电穿孔技术过表达或减少miR-9，然后通过mRNA原位杂交和免疫荧光染色来评估miR-9的表达以及改变miR-9表达的影响。我们研究了miR-9在鸡中后脑区（MH）早期发育过程中的表达和功能。我们的分析揭示了鸡miR-9与哺乳动物miR-9的关系比与鱼的关系更密切，并且在鸡神经管中具有动态表达模式。在发育早期，miR-9在整个大脑中弥漫性表达，但前脑除外，并且在后期阶段它变得更加局限于CNS的特定区域。miR-9在HH 9 -10的过表达导致FGF 8表达的减少和中脑-后脑边界（MHB）中的过早神经元分化。在中脑内，miR-9不会引起神经元的过早分化，而是减少了在midbrain.Conclusion增殖：我们的研究结果表明，miR-9具有区域特异性的影响，在发展中的中后脑区域与分歧的区域祖细胞的反应。
Background: MiR-9 is a small non-coding RNA that is highly conserved between species and primarily expressed in the central nervous system (CNS). It is known to influence proliferation and neuronal differentiation in the brain and spinal cord of different vertebrates. Different studies have pointed to regional and species-specific differences in the response of neural progenitors to miR-9.Methods: In ovo and ex ovo electroporation was used to overexpress or reduce miR-9 followed by mRNA in situ hybridisation and immunofluorescent stainings to evaluate miR-expression and the effect of changed miR-9 expression.Results: We have investigated the expression and function of miR-9 during early development of the mid-hind-brain region (MH) in chick. Our analysis reveals a closer relationship of chick miR-9 to mammalian miR-9 than to fish and a dynamic expression pattern in the chick neural tube. Early in development, miR-9 is diffusely expressed in the entire brain, bar the forebrain, and it becomes more restricted to specific areas of the CNS at later stages. MiR-9 overexpression at HH9-10 results in a reduction of FGF8 expression and premature neuronal differentiation in the mid-hindbrain boundary (MHB). Within the midbrain miR-9 does not cause premature neuronal differentiation it rather reduces proliferation in the midbrain.Conclusion: Our findings indicate that miR-9 has regional specific effects in the developing mid-hindbrain region with a divergence of response of regional progenitors.