Increase of fecundity by probiotic administration in zebrafish (Danio rerio)
Increase of fecundity by probiotic administration in zebrafish (Danio rerio)
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DOI：
10.1530/rep-10-0145
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发表时间：
2010-12-01
期刊：
影响因子：
3.8
通讯作者：
Carnevali, Oliana
中科院分区：
文献类型：
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作者：
Gioacchini, Giorgia;Maradonna, Francesca;Carnevali, Oliana
众所周知，生殖对能量储备的状态很敏感，能量平衡和生育之间存在平衡。因此，本研究探讨了益生菌鼠李糖乳杆菌作为饲料添加剂对斑马鱼(Danio Rerio)繁殖力的影响。10天的益生菌治疗调节神经肽激素和代谢信号的基因表达，如中枢和外周水平的Kiss1、Kiss2和Leptin。体内排卵量和体外成熟试验获得的生发泡破碎率均较高，说明益生菌提高了受精率。卵母细胞成熟度的增加与编码信号的基因如lhcgr、cbr11和paqr8的转录增加有关，同时伴随着编码阻止卵母细胞成熟的局部因子的基因的转录减少，如tgfb1、gdf9和bmp15。综上所述，所有这些发现都突显了鼠李糖对内分泌系统和局部水平通过诱导卵母细胞成熟所起的作用。本研究结果的重要性强调了饲料在生殖过程中的重要性，支持了饲料添加剂可以提高繁殖力的假说。考虑到斑马鱼已经被明确地确立为生物医学研究的脊椎动物模型，这些结果支持了经常在人类饮食中使用的益生菌等饲料添加剂作为一种新技术来改善包括人类在内的所有脊椎动物的生殖的潜力。生殖(2010)140 953-959
It is well known established that reproduction is sensitive to the state of energy reserves, and that there is a balance between energy homeostasis and fertility. In this view, this study examined the effects of the probiotic Lactobacillus rhamnosus, as a feed additive, on zebrafish (Danio rerio) fecundity. Ten days of probiotic treatment modulate the gene expression of neuropeptide hormones and metabolic signals, such as kiss1, kiss2 and leptin both at the CNS level and at the peripheral level. The increase in fecundity brought about by the probiotic was demonstrated by the higher number of ovulated eggs in vivo and by the higher germinal vesicle breakdown rate obtained with the in vitro maturation assay. The increase in oocyte maturation was associated with increased transcription of genes coding for signals which induce the maturation phase, such as lhcgr, cbr1l, and paqr8 genes, concomitant with a decreased transcription of genes coding for local factors which prevent oocyte maturation, such as tgfb1, gdf9, and bmp15. In conclusion, all these findings highlight the action of L. rhamnosus both on the endocrine system and at the local level by inducing oocyte maturation. The significance of the results herein obtained underlined the importance of diet in the reproductive process, supporting the hypothesis that feed additives can improve fecundity. Considering that the zebrafish has been clearly established as a vertebrate model for biomedical research, these results support the potentiality of feed additives such as probiotics, frequently used in the human diet, as a new technology to improve reproduction in all vertebrates, including humans. Reproduction (2010) 140 953-959