DNA copy number losses in chromosome 14: an early change in gastrointestinal stromal tumors.
DNA copy number losses in chromosome 14: an early change in gastrointestinal stromal tumors.
复制标题
14 号染色体 DNA 拷贝数丢失：胃肠道间质瘤的早期变化。
作者：
W. El‐Rifai;M. Sarlomo‐Rikala;Markku Miettinen;Sakari Knuutila;Leif C. Andersson
通过比较基因组杂交研究了 13 例恶性、3 例交界性和 16 例良性胃肠道间质瘤 (GIST) 的 DNA 拷贝数变化。一致的发现是染色体 14q 中 DNA 拷贝数的丢失。在 75% 的良性肿瘤、62% 的恶性肿瘤以及位于 14q22 的最小重叠区域的三个边界肿瘤中的两个中检测到了这一点。 DNA 拷贝数的丢失比增加更常见，恶性 GIST 中有 2-10 个变化，良性肿瘤中有 1-3 个变化。仅在恶性 GIST 中检测到的高水平 DNA 扩增被分配到 3q26-q29 (40%)、5p (30%) 和 8q22-24 (40%)。我们的结果表明，14q22 中的拷贝数丢失是 GIST 肿瘤发生过程中的早期变化，并表明 14q22 上的新肿瘤抑制基因可能参与此类间充质细胞的分化或增殖的调节。
The DNA copy number changes were investigated in 13 malignant, 3 borderline, and 16 benign gastrointestinal stromal tumors (GISTs) by comparative genomic hybridization. A consistent finding was the loss of DNA copy numbers in chromosome 14q. This was detected in 75% of the benign tumors, in 62% of the malignant tumors, and in two out of the three borderline tumors with a minimal overlapping region located to 14q22. Losses of DNA copy numbers were more frequent than gains, with 2-10 changes in malignant GISTs and 1-3 changes in benign tumors. High-level DNA amplifications, detected only in malignant GISTs, were assigned to 3q26-q29 (40%), 5p (30%), and 8q22-24 (40%). Our results indicate that copy number losses in 14q are an early change during oncogenesis of GISTs and suggest the possibility that a new tumor suppressor gene at 14q22 might be involved in the regulation of differentiation or proliferation of such mesenchymal cells.