A fish protein hydrolysate alters fatty acid composition in liver and adipose tissue and increases plasma carnitine levels in a mouse model of chronic inflammation
A fish protein hydrolysate alters fatty acid composition in liver and adipose tissue and increases plasma carnitine levels in a mouse model of chronic inflammation
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DOI：
10.1186/1476-511x-12-143
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发表时间：
2013-10-07
影响因子：
4.5
通讯作者：
Berge, Rolf K.
中科院分区：
文献类型：
--
作者：
Bjorndal, Bodil;Berge, Christ;Berge, Rolf K.
背景：越来越多的证据表明，鱼蛋白水解物（FPH）饮食影响动物线粒体脂肪酸代谢。本研究的目的是确定FPH是否可以影响表达人肿瘤坏死因子α（hTNF α）的转基因小鼠中的脂肪酸代谢和炎症。方法：hTNF α小鼠（C57 BL/6 hTNF α）被给予含有20%酪蛋白（对照组）或15%FPH和5%酪蛋白（FPH组）的高脂肪（23%，w/w）饮食两周。禁食过夜后，采集血液、脂肪组织和肝脏样本。分析肝脏中的基因表达和酶活性，分析肝脏和卵巢白色脂肪组织中的脂肪酸组成，并分析血浆中的炎症参数、肉毒碱和酰基肉毒碱。在脂肪组织和肝脏中，喂食FPH饮食的小鼠中的n-3/n-6脂肪酸比高于喂食对照饮食的小鼠，FPH饮食影响了0.6和0.9去饱和酶的基因表达。喂食这种饮食的小鼠也表现出较低的脂肪酸合成酶的肝脏活性。同时，观察到较低的血浆INF-γ水平。血浆肉毒碱和肉毒碱前体γ-丁酰甜菜碱是较高的FPH组相比，对照组，血浆短链和中链酰基肉毒碱酯。较高水平的血浆乙酰肉毒碱可能反映了刺激的线粒体和过氧化物酶体β-氧化的脂肪酸，过氧化物酶体酰基辅酶A氧化酶1和线粒体肉毒碱棕榈酰转移酶-II的肝脏活动较高的FPH喂养mice.Conclusions：FPH饮食被证明影响肝脏脂肪酸代谢和脂肪酸组成。这表明对脂肪酸代谢的影响对于海洋来源的蛋白质水解物的生物活性是重要的。
Background: There is growing evidence that fish protein hydrolysate (FPH) diets affect mitochondrial fatty acid metabolism in animals. The aim of the study was to determine if FPH could influence fatty acid metabolism and inflammation in transgene mice expressing human tumor necrosis factor alpha (hTNF alpha).Methods: hTNF alpha mice (C57BL/6 hTNFa) were given a high-fat (23%, w/w) diet containing 20% casein (control group) or 15% FPH and 5% casein (FPH group) for two weeks. After an overnight fast, blood, adipose tissue, and liver samples were collected. Gene expression and enzyme activity was analysed in liver, fatty acid composition was analyzed in liver and ovarian white adipose tissue, and inflammatory parameters, carnitine, and acylcarnitines were analyzed in plasma.Results: The n-3/n-6 fatty acid ratio was higher in mice fed the FPH diet than in mice fed the control diet in both adipose tissue and liver, and the FPH diet affected the gene expression of.6 and.9 desaturases. Mice fed this diet also demonstrated lower hepatic activity of fatty acid synthase. Concomitantly, a lower plasma INF-gamma level was observed. Plasma carnitine and the carnitine precursor gamma-butyrobetaine was higher in the FPH-group compared to control, as was plasma short-chained and medium-chained acylcarnitine esters. The higher level of plasma acetylcarnitine may reflect a stimulated mitochondrial and peroxisomal beta-oxidation of fatty acids, as the hepatic activities of peroxisomal acyl-CoA oxidase 1 and mitochondrial carnitine palmitoyltransferase-II were higher in the FPH-fed mice.Conclusions: The FPH diet was shown to influence hepatic fatty acid metabolism and fatty acid composition. This indicates that effects on fatty acid metabolism are important for the bioactivity of protein hydrolysates of marine origin.