Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and Δ12-PGJ2
Sox-4 is a positive regulator of Hep3B and HepG2 cells' apoptosis induced by prostaglandin (PG)A2 and Δ12-PGJ2
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DOI：
10.1038/emm.2002.34
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发表时间：
2002-07-31
影响因子：
12.8
通讯作者：
Kim, IK
中科院分区：
文献类型：
--
作者：
Ahn, SG;Kim, HS;Kim, IK
我们早些时候报道了前列腺素A2和Delta(12)-PGJ(2)诱导人肝癌细胞株Hep3B细胞凋亡过程中SOX-4的表达上调。在这项研究中，SOX-4在人Hep3B和HepG2细胞系中的作用被检测。A23187(钙离子载体)和依托泊苷(拓扑异构酶II抑制剂)等几种凋亡诱导剂对SOX-4的诱导作用以及通过细胞DNA片段化观察到的SOX-4基因均能诱导细胞发生凋亡。SOX-4反义寡核苷酸抑制PGA(2)和(12)-PGJ(2)诱导的SOX-4表达，阻断PGA(2)和(12)-PGJ(2)诱导的DNA片段化。SOX-4诱导的细胞凋亡伴随着caspase-1的激活，表明caspase级联通路参与了这一凋亡通路。这些结果表明，SOX-4作为一个重要的凋亡介质参与了Hep3B和HepG2细胞的凋亡。
We reported earlier that expression of Sox-4 was found to be elevated during prostaglandin (PG) A2 and Delta(12)-PGJ(2) induced apoptosis in human hepatocarcinoma Hep3B cells. In this study, the role of Sox-4 was examined using human Hep3B and HepG2 cell lines. Sox-4 induction by several apoptotic inducer such as A23187 (Ca2+ ionophore) and etoposide (topoisomerase II inhibitor) and Sox-4 transfection into the cells were able to induce apoptosis as observed by the cellular DNA fragmentation. Antisense oligonucleotide of Sox-4 inhibited the induction of Sox-4 expression and blocked the formation of DNA fragmentation by PGA(2) and (12)-PGJ(2) in Hep3B and HepG2 cells. Sox-4-induced apoptosis was accompanied with caspase-1 activation indicating that caspase cascade was involved in this apoptotic pathway.These results indicate that Sox-4 is involved in Hep3B and HepG2 cells apoptosis as an important apoptotic mediator.