Influence of intercellular tissue connections on airway muscle mechanics
Influence of intercellular tissue connections on airway muscle mechanics
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DOI：
10.1152/jappl.1999.86.1.5
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发表时间：
1999-01-01
影响因子：
3.3
通讯作者：
Meiss, RA
中科院分区：
文献类型：
--
作者：
Meiss, RA
内脏器官中平滑肌的收缩受到肌肉外部结构的改变。在肌肉组织本身内，结缔组织在收缩细胞之间的力传递中起着重要作用。径向排列的连接可以通过限制短长度的组织扩张来影响收缩力学。先前的研究表明，极端缩短时刚度的增加是由于这种径向约束。报告了进一步研究这些影响的两种方法。为了增加径向约束，将非常薄的硅橡胶带松散地放置在静止长度的犬气管肌条周围。使条带在轻后载荷下缩短，膨胀直至被带子限制。随后去除带子允许增加缩短，并且在短长度下刚度增加较少。观察到相关的等距效应。为了减少限制，用胶原酶部分消化肌肉条。与对照条件相比，这种处理允许进一步缩短，并且短长度下的刚度增加较少。这些结果强调了细胞外结构在确定平滑肌机械功能中的作用。
Contraction of smooth muscle in visceral organs is modified by structures external to the muscle. Within muscle tissue itself, connective tissue plays an important role in force transference among the contractile cells. Connections arranged radially can affect contractile mechanics by limiting tissue expansion at short lengths. Previous work suggests that increased stiffness at extreme shortening is due to such radial constraints. Two approaches to further study of these effects are reported. To increase radial constraints, very thin Silastic bands were placed loosely about strips of canine trachealis muscle at rest length. The strips were allowed to shorten under light afterloads, expanding until restrained by the bands. Subsequent removal of the bands allowed increased shortening, with less increase in stiffness at short lengths. Related isometric effects were observed. To reduce constraints, muscle strips were partially digested with collagenase. Compared with control conditions, this treatment permitted further shortening, with less increase in stiffness at short lengths. These results emphasize the role of extracellular structures in determining mechanical function of smooth muscle.