HUMAN PAPILLOMAVIRUS TYPE-6 AND TYPE-11 HAVE ANTIGENICALLY DISTINCT STRONGLY IMMUNOGENIC CONFORMATIONALLY DEPENDENT NEUTRALIZING EPITOPES
HUMAN PAPILLOMAVIRUS TYPE-6 AND TYPE-11 HAVE ANTIGENICALLY DISTINCT STRONGLY IMMUNOGENIC CONFORMATIONALLY DEPENDENT NEUTRALIZING EPITOPES
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DOI：
10.1006/viro.1994.1649
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发表时间：
1994-11-15
期刊：
影响因子：
3.7
通讯作者：
KREIDER, JW
中科院分区：
文献类型：
--
作者：
CHRISTENSEN, ND;KIRNBAUER, R;KREIDER, JW
在ELISA和HPV-11中和试验中测试对HPV 6型和11型反应的抗体，以确定这些密切相关的类型是否共享交叉反应性中和表位。使用完整的HPV-6 L1 VLP检测了一系列针对感染性HPV-11和HPV-11 L1病毒样颗粒（VLP）上构象表位的HPV-11中和单克隆抗体（N-MAb）的反应性类型特异性。还使用无胸腺小鼠异种移植系统测试针对完整HPV-6 L1 VLP产生的多克隆抗血清的HPV-11中和能力。结果表明，HPV-11上的构象依赖性中和表位具有很强的型特异性。四种HPV-11 N-MAb中的三种与HPV-6 L1 VLP的结合为阴性，第四种与HPV-6 L1 VLP的结合比其与HPV-11 L1 VLP的结合弱几个数量级。即使在1：100的低稀释度下，多克隆抗HPV-6 L1 VLP抗血清也仅对HPV-11感染性具有部分保护作用。相比之下，多克隆抗HPV-11 L1 VLP抗血清在大于1：10，000的稀释度下具有完全保护性。(C)1994年出版社出版。
Antibodies reactive to HPV types 6 and 11 were tested in ELISA and HPV-11 neutralization assays to determine whether these closely related types shared cross-reactive neutralizing epitopes. A series of HPV-11 neutralizing monoclonal antibodies (N-MAbs) that targeted conformational epitopes on infectious HPV-11 and HPV-11 L1 virus-like particles (VLPs) were tested for type-specificity of reactivity using intact HPV-6 L1 VLPs. Polyclonal antisera generated against intact HPV-6 L1 VLPs were also tested for HPV-11 neutralizing capacity using the athymic mouse xenograft system. The results demonstrated that conformationally dependent neutralizing epitopes on HPV-11 were Very type-specific. Three of the four HPV-11 N-MAbs were negative for binding to HPV-6 L1 VLP, and the fourth demonstrated binding to HPV-6 L1 VLPs that was several orders of magnitude weaker than its binding to HPV-11 L1 VLP. The polyclonal anti-HPV-6 L1 VLP antiserum was only partially protective against HPV-11 infectivity even at a low dilution of 1:100. In contrast, polyclonal anti-HPV-11 L1 VLP antiserum was completely protective at dilutions greater than 1:10,000. (C) 1994 Academic Press, Inc.