A rolB regulatory factor belongs to a new class of single zinc finger plant proteins
A rolB regulatory factor belongs to a new class of single zinc finger plant proteins
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DOI：
10.1046/j.1365-313x.1996.10020215.x
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发表时间：
1996-08-01
期刊：
影响因子：
7.2
通讯作者：
Capone, I
中科院分区：
文献类型：
--
作者：
DePaolis, A;Sabatini, S;Capone, I
一种与植物癌基因rolB在（根）分生组织中表达所必需的调节结构域B结合的蛋白质含有双子叶植物和单子叶植物中保守的新型的单个锌指。条带移动分析显示在烟草细胞核中存在选择性结合结构域B的蛋白质，其四聚体用于从烟草表达文库中分离cDNA（NtBBF 1，Nicotiana tabacum rolB结构域B因子1），相应的基因组克隆也被分离。NtBBF 1编码的蛋白含有一个C2 C2锌指结构，其靶序列位于结构域B。锌指的DNA结合能力通过将后者与谷胱甘肽-S-转移酶蛋白融合来评估，所述谷胱甘肽-S-转移酶蛋白显示与NtBBF 1结合相同的靶序列。还分离了许多其他烟草cDNA，其编码具有包含与NtBBF 1相同的锌指的结构域（BBF结构域）的不同蛋白。此外，从拟南芥中分离了编码具有几乎相同的单个锌指的蛋白质的cDNA。最近在玉米转录因子中发现了一个非常密切相关的锌指，称为Dof结构域。烟草、拟南芥和玉米的BBF/Dof结构域蛋白是一个新的广泛的植物转录因子家族的成员，该家族通过在植物界广泛使用的单个锌指起作用。
A protein which binds to the regulatory domain B necessary for expression of the plant oncogene rolB in (root) meristems contains a single zinc finger of a novel type conserved in dicots and monocots. Band shift analysis revealed the presence in tobacco nuclei of a protein selectively binding to domain B, a tetramer of which was used to isolate a cDNA (NtBBF1, Nicotiana tabacum rolB domain B factor 1) from a tobacco expression library, The corresponding genomic clone was also isolated. The protein encoded by NtBBF1 contains a single C2C2 zinc finger, and its target sequence in domain B was identified by means of mutagenized oligonucleotides. The DNA-binding capability of the zinc finger was assessed by means of a fusion of this latter with the glutathione-S-transferase protein, that was shown to bind the same target sequence as NtBBF1. A number of other tobacco cDNAs encoding different proteins with a domain (BBF domain) encompassing the zinc finger identical to NtBBF1 were also isolated. Furthermore, a cDNA encoding a protein with an almost identical single zinc finger was isolated from Arabidopsis. A very closely related zinc finger has very recently been identified in maize transcription factors and termed the Dof domain. It is proposed that the tobacco, Arabidopsis and maize BBF/Dof domain proteins are members of a new broad family of plant transcription factors acting through a single zinc finger widely utilized in the plant kingdom.