MISREADING OF THE RIBOSOMAL SUPPRESSOR SUP46 DUE TO AN ALTERED 40S SUBUNIT IN YEAST
MISREADING OF THE RIBOSOMAL SUPPRESSOR SUP46 DUE TO AN ALTERED 40S SUBUNIT IN YEAST
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DOI：
10.1016/0022-2836(81)90490-3
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发表时间：
1981-01-01
影响因子：
5.6
通讯作者：
SHERMAN, F
中科院分区：
文献类型：
--
作者：
MASUREKAR, M;PALMER, E;SHERMAN, F
在酵母S.酿酒酵母作用于广泛的不同类型的突变。在用poly（U）编程的无细胞系统中苯丙氨酸的掺入和亮氨酸的错误掺入表明来自SUP 46菌株的核糖体产生异常高的翻译错误率。使用无细胞翻译系统证明SUP46缺陷存在于40 S核糖体亚基中。SUP46菌株的生长对巴龙霉素（一种诱导翻译错误的氨基糖苷类抗生素）异常敏感。此外，巴龙霉素刺激错误翻译与SUP46核糖体在更大程度上比正常的核糖体。SUP46抑制是由40 S核糖体亚基突变改变导致的翻译错误增加引起的。巴龙霉素显然在SUP46菌株中产生翻译错误，其速率对于细胞生长来说太高。
The dominant suppressor, SUP46, in the yeast S. cerevisiae acts on a wide range of different types of mutations. The incorporation of phenylalanine and the misincorporation of leucine in a cell-free system programmed with poly(U) indicated that the ribosomes from a SUP46 strain produce abnormally high rates of translation errors. The cell-free translation system was used to demonstrate that the SUP46 defect resides in the 40 S ribosomal subunit. The growth of SUP46 strains was unusually sensitive to paromomycin, an aminoglycoside antibiotic that induces translation errors. In addition, paromomycin stimulted mistranslation with SUP46 ribosomes to a greater extent than with normal ribosomes. SUP46 suppression is caused by increased translation errors as a result of the mutationally altered 40 S ribosomal subunit. Paromomycin apparently produces translation errors in SUP46 strains at rates that are too high for cellular growth.