Inhibition of glycoprotein and glycolipid synthesis in hamster embryo cells by cytosine arabinoside and hydroxyurea
Inhibition of glycoprotein and glycolipid synthesis in hamster embryo cells by cytosine arabinoside and hydroxyurea
复制标题
阿糖胞苷和羟基脲对仓鼠胚胎细胞糖蛋白和糖脂合成的抑制作用
作者：
A. Hawtrey;T. Scott‐Burden;G. Robertson
已知核苷1-β- d -阿拉伯糖醛酸胞嘧啶（ara-C）和不相关的化合物羟基脲可以抑制细菌和动物细胞中的DNA复制1- 3。阿拉伯糖脲基胞苷-5 ' -三磷酸（ara-CTP）特异性地干扰细菌细胞提取物中DNA聚合酶II的作用，而羟基脲则影响核糖核苷二磷酸还原酶反应。我们已经证明ara-C和羟基脲能在组织培养中促进仓鼠胚胎细胞的转化。此外，研究还表明，ara-C可以抑制3h - d -氨基葡萄糖（n -乙酰神经氨酸的前体）在仓鼠胚胎成纤维细胞和转化细胞中与糖蛋白和糖脂的结合7。在继续这些实验中，我们感兴趣的是，首先，确定ara-C在细胞中的作用位点（可能是它的三磷酸），其次，研究DNA复制的其他抑制剂对糖蛋白和糖脂合成的可能影响。
THE nucleoside, 1-β-D-arabinofuranosylcytosine (ara-C) and the unrelated compound, hydroxyurea, are known to inhibit DNA replication in bacterial and animal cells1–3. Arabinofuranosyl cytidine-5′-triphosphate (ara-CTP) specifically interferes with the action of DNA polymerase II in bacterial cell extracts4, whereas hydroxyurea affects the ribonucleoside diphosphate reductase reaction5. We have shown that ara-C and hydroxyurea bring about transformation of hamster embryo cells in tissue culture6. Further, it has also been demonstrated that ara-C inhibits the incorporation of 3H-D-glucosamine (a precursor of N-acetyl-neuraminic acid) into glycoproteins and glycolipids of hamster embryo fibroblasts and transformed cells7. In continuing these experiments we have been interested in, first, determining the site or sites of action of ara-C (presumably as its triphosphate) in the cell and second, studying other inhibitors of DNA replication as to their possible effect on glycoprotein and glycolipid synthesis.