Effect of Lesion Baseline Severity and Mineral Distribution on Remineralization and Progression of Human and Bovine Dentin Caries Lesions
Effect of Lesion Baseline Severity and Mineral Distribution on Remineralization and Progression of Human and Bovine Dentin Caries Lesions
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DOI：
10.1159/000431039
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发表时间：
2015-01-01
期刊：
影响因子：
4.2
通讯作者：
Lynch, Richard J.
中科院分区：
文献类型：
--
作者：
Lippert, Frank;Churchley, David;Lynch, Richard J.
本实验室研究的目的是比较病变的基线严重程度，矿物质分布和基板上的再矿化和在根牙本质中产生的龋齿病变的进展的影响。使用三种方案在由人和牛牙本质制备的牙本质标本中形成病变，每种方案均利用三个脱矿时间段来创建不同矿物分布（表面下、中度软化、极度软化）的病变，并且在每种病变类型内具有不同的严重程度。然后将病变进一步矿化或脱矿，并使用横向显微放射照相术进行分析。在病变基线，没有发现人类和牛牙本质之间的综合矿物质损失（Δ Z）的差异。病变类型之间的矿物质分布差异明显。人牙本质病变比牛牙本质病变更容易发生继发性脱矿（Delta Delta Z），尽管Delta L没有差异。同样，较小的病变比较大的病变更容易发生继发性脱矿。皮下病变比中度和极度软化的病变更耐酸。再矿化后，人和牛牙本质病变之间的差异并不明显的Δ Δ Z，虽然牛牙本质病变表现出更大的减少病变深度L。对于病变类型，对再矿化的反应性（Δ Δ Z）的顺序为极度软化>中度软化>皮下。更多的脱矿病变表现出更大的矿化比浅。总之，人类和牛牙本质之间存在一些差异，它们对去矿化和再矿化的相对反应性也存在差异。然而，这些差异被病变基线矿物质分布和严重程度的影响所掩盖。因此，牛牙本质似乎是一个合适的替代人类牙本质的机械根龋研究。(C)2015 S. Karger AG，巴塞尔
The aims of this laboratory study were to compare the effects of lesion baseline severity, mineral distribution and substrate on remineralization and progression of caries lesions created in root dentin. Lesions were formed in dentin specimens prepared from human and bovine dentin using three protocols, each utilizing three demineralization periods to create lesions of different mineral distributions (subsurface, moderate softening, extreme softening) and severity within each lesion type. Lesions were then either remineralized or demineralized further and analyzed using transverse microradiography. At lesion baseline, no differences were found between human and bovine dentin for integrated mineral loss (Delta Z). Differences in mineral distribution between lesion types were apparent. Human dentin lesions were more prone to secondary demineralization (Delta Delta Z) than bovine dentin lesions, although there were no differences in Delta L. Likewise, smaller lesions were more susceptible to secondary demineralization than larger ones. Subsurface lesions were more acid-resistant than moderately and extremely softened lesions. After remineralization, differences between human and bovine dentin lesions were not apparent for Delta Delta Z although bovine dentin lesions showed greater reduction in lesion depth L. For lesion types, responsiveness to remineralization (Delta Delta Z) was in the order extremely softened > moderately softened > subsurface. More demineralized lesions exhibited greater remineralization than shallower ones. In summary, some differences exist between human and bovine dentin and their relative responsiveness to de- and remineralization. These differences, however, were overshadowed by the effects of lesion baseline mineral distribution and severity. Thus, bovine dentin appears to be a suitable substitute for human dentin in mechanistic root caries studies. (C) 2015 S. Karger AG, Basel