Characterization of phenolic compounds in wild medicinal flowers from Portugal by HPLC-DAD-ESI/MS and evaluation of antifungal properties
Characterization of phenolic compounds in wild medicinal flowers from Portugal by HPLC-DAD-ESI/MS and evaluation of antifungal properties
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DOI：
10.1016/j.indcrop.2012.11.003
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发表时间：
2013-01-01
影响因子：
5.9
通讯作者：
Ferreira, Isabel C. F. R.
中科院分区：
文献类型：
--
作者：
Barros, Lillian;Alves, Carlos Tiago;Ferreira, Isabel C. F. R.
本文采用HPLC-DAD-ESI/MS法对葡萄牙东北部板栗花、榆叶梅花和薇甘菊花中的酚类化合物进行了表征，并对其抗念珠菌活性进行了初步筛选（白色念珠菌，光滑念珠菌，近平滑假丝酵母和热带假丝酵母）。C sativa样品中酚类化合物的含量最高（18973 +/- 40 μ g/g，fw）和可水解单宁（14873 +/- 110 μ g/g）。酚酸（569 +/- 20 μ g/g）和类黄酮（6090 +/- 253 μ g/g）的最高含量在F. ulmaria和R.薇甘菊样品。水解单宁（如三和二别戊酰HHDP葡萄糖）是苜蓿和苜蓿中酚类化合物的主要类群。而黄酮类化合物（如（+）-儿茶素和原花青素二聚体和三聚体）在黄榆中含量最高。薇甘菊因此，后者对所有念珠菌属物种显示出更强的作用（MIC
In the present work, the phenolic compounds of Castanea sativa, Filipendula ulmaria and Rosa micrantha flowers from Northeastern Portugal were characterized by HPLC-DAD-ESI/MS. Furthermore, it was performed a screening of their antifungal potential against Candida species (Candida albicans, Candida glabrata, Candida parapsilosis and Candida tropicalis).C sativa sample gave the highest amount of phenolic compounds (18973 +/- 40 mu g/g, fw) and hydrolysable tannins (14873 +/- 110 mu g/g). The highest amounts of phenolic acids (569 +/- 20 mu g/g) and flavonoids (6090 +/- 253 mu g/g) were obtained in F. ulmaria and R. micrantha samples, respectively. Hydrolysable tannins (e.g. tri and digalloyl HHDP glucose) were the main group of phenolic compounds in C sativa and F. ulmaria samples, while flavonoids (e.g. (+)-catechin and procyanidin dimers and trimers) were the most abundant group in R. micrantha. Thus, the stronger effect showed by this latter against all the Candida species (MIC