CHARACTERIZATION OF OSCILLATIONS OF INTRACELLULAR CALCIUM-CONCENTRATION IN FERRET VENTRICULAR MUSCLE
CHARACTERIZATION OF OSCILLATIONS OF INTRACELLULAR CALCIUM-CONCENTRATION IN FERRET VENTRICULAR MUSCLE
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DOI：
10.1113/jphysiol.1984.sp015281
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发表时间：
1984-01-01
影响因子：
5.5
通讯作者：
ORCHARD, CH
中科院分区：
文献类型：
--
作者：
ALLEN, DG;EISNER, DA;ORCHARD, CH
将发光蛋白水母发光蛋白注入雪貂乳头肌表层细胞。监测张力和水母发光蛋白（细胞内[Ca 2 +]的功能）。通过降低细胞外Na+或毒毛旋花子苷抑制Na泵来增加细胞内Ca ~（2+）浓度（[Ca ~（2+）]i），可产生[Ca ~（2+）]i和张力的自发振荡。傅立叶分析表明，这些振荡的频率高达3-4赫兹。如果在这些条件下刺激肌肉，与抽搐相关的Ca瞬变之后是一系列[Ca 2 +]i的阻尼振荡，其伴随着后收缩。在给定的条件下，受激振荡的频率与自发振荡的频率相似。机动，增加[Ca 2 +]i增加的自发和受激振荡的频率。抑制肌浆网功能的药物（咖啡因和ryanodine）消除了受激振荡和自发振荡。无钠挛缩过程中的自发振荡不受钙通道阻滞剂D-600的影响。当重复刺激开始时，受激振荡的频率和幅度在几分钟内增加。增加刺激的频率增加了受激振荡的幅度。[Ca ~（2+）]i的自发振荡可能是由于肌浆网振荡性Ca释放所致。自发振荡和受激振荡的相似性质表明，后者可能是由于前者的同步。
The photoprotein aequorin was injected into superficial cells of ferret papillary muscles. Tension and aequorin light (a function of intracellular [Ca2+]) were monitored. Increasing intracellular Ca concentration ([Ca2+]i) either by decreasing extracellular Na, or by inhibiting the Na pumps with strophanthidin, produced spontaneous oscillations of [Ca2+]i and tension. Fourier analysis showed that these oscillations had frequencies of up to 3-4 Hz. If the muscle was stimulated in these conditions, the Ca transient associated with the twitch was followed by a series of damped oscillations of [Ca2+]i which were accompanied by after-contractions. Under a given set of conditions the frequency of the stimulated oscillations was similar to that of the spontaneous oscillations. Maneuvers which increase [Ca2+]i increased the frequency of both spontaneous and stimulated oscillations. Drugs which inhibit the function of the sarcoplasmic reticulum (caffeine and ryanodine) abolished both stimulated and spontaneous oscillations. The spontaneous oscillations during a Na-free contracture were unaffected by the Ca channel blocker D-600. When repetitive stimulation was begun the frequency and magnitude of the stimulated oscillations increased over several minutes. Increasing the frequency of stimulation increased the magnitude of the stimulated oscillations. The spontaneous oscillations of [Ca2+]i may be due to oscillatory Ca release from the sarcoplasmic reticulum. The similar properties of the spontaneous and stimulated oscillations suggest that the latter may be due to a synchronization of the former.